Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015024_tpc0 GATTCACTACAGTGTGAGCAACTCGGCTCTTG(+ACTCAC)GATCCAAAGCAGCACTTCATATATAAAAGGG NGG 10 8 17 8 24 0 100 5 10
    240605015023_o376 GTTTGCCCTTTTATATATGAAGTGCTGCTTTG(+ACTCAC)GATCCAAGAGCCGAGTTGCTCACACTGTAGT NGG 10 8 17 8 24 0 100 5 10
    240605015022_qran GACCCACAGGAACTATGAAGGAAGAAGCGTAG(+ACTCAC)GATCCCAGAGCAATCATATAACCGAACTCTG NGG 10 8 17 8 24 0 100 5 10
    240605015020_5v06 CGGTTCAGAGTTCGGTTATATGATTGCTCTGG(+ACTCAC)GATCCTACGCTTCTTCCTTCATAGTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605015019_eij6 TCTTCGCCCTACTAAGCCATTCCCATATGTAG(+ACTCAC)GATCCTTCTTCTTGTCAGAAGATCAAATTAC NGG 10 8 17 8 24 0 100 5 10
    240605015018_auxy TTTAAGTAATTTGATCTTCTGACAAGAAGAAG(+ACTCAC)GATCCTACATATGGGAATGGCTTAGTAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605015017_9kp1 AAGGAGAGTGTTCAGGCAATATAATTGATCTG(+ACTCAC)GTACCAGGGTTCAAGGGTGAATGGTGGGTGC NGG 10 8 17 8 24 0 100 5 10
    240605015014_ueuy TCTTTGCACCCACCATTCACCCTTGAACCCTG(+ACTCAC)GTACCAGATCAATTATATTGCCTGAACACTC NGG 10 8 17 8 24 0 100 5 10
    240605015013_fgdq AGGGGAGGGTTAGTTCTATCACCTTTTTTATG(+ACTCAC)GTACCTGATTGCTTTGCTTATCCATCAACAG NGG 10 8 17 8 24 0 100 5 10
    240605015012_79lk GAGCACTGTTGATGGATAAGCAAAGCAATCAG(+ACTCAC)GTACCATAAAAAAGGTGATAGAACTAACCCT NGG 10 8 17 8 24 0 100 5 10