Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014929_cfo0 CACTGACAAGTGATATTAGCCATAAAGTGCAG(+ACTCAC)GTACCATGCCACACTCCACAGACCAAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605014927_yozv TTTCACCTCCTTGGTCTGTGGAGTGTGGCATG(+ACTCAC)GTACCTGCACTTTATGGCTAATATCACTTGT NGG 10 8 17 8 24 0 100 5 10
    240605014925_9wui TGTTGCAGGGCTATAGGAAGACAAAGCCTGGG(+ACTCAC)GTACCCGCATCTGGTTTGGAACCCTGAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605014923_c9pn CAACAAAACTCAGGGTTCCAAACCAGATGCGG(+ACTCAC)GTACCCCAGGCTTTGTCTTCCTATAGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605014921_kwuc TTTTCTGTTTCACATCTTGTTTTTAAAAATAG(+ACTCAC)GATCCAAAACATCTCAAATCATCTCCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605014920_v61g ATTCTGTTAAGGAGATGATTTGAGATGTTTTG(+ACTCAC)GATCCTATTTTTAAAAACAAGATGTGAAACA NGG 10 8 17 8 24 0 100 5 10
    240605014919_ir5i GTTTGTTATCATAGTTGGTCAATGCCTGGCAG(+ACTCAC)GTACCAGCATTTTGTCACTTGGGTCAAACCA NGG 10 8 17 8 24 0 100 5 10
    240605014917_eifb TCCCTTGGTTTGACCCAAGTGACAAAATGCTG(+ACTCAC)GTACCTGCCAGGCATTGACCAACTATGATAA NGG 10 8 17 8 24 0 100 5 10
    240605014913_ii5k TCCTAACTGCTGAGCCATCTCTCCAGCCCCGG(+ACTCAC)GTACCACAGTATTTGAAACCACAAAAATCAG NGG 10 8 17 8 24 0 100 5 10
    240605014912_9i5e CATCACTGATTTTTGTGGTTTCAAATACTGTG(+ACTCAC)GTACCCGGGGCTGGAGAGATGGCTCAGCAGT NGG 10 8 17 8 24 0 100 5 10