Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014823_2vvb GCTTTGGTATAGTGAATGTCTCCCTGTGCAGG(+ACTCAC)GTACCACGCCATGTGCCATTTAGGCACAAGC NGG 10 8 17 8 24 0 100 5 10
    240605014822_774z GTCTGGCTTGTGCCTAAATGGCACATGGCGTG(+ACTCAC)GTACCCTGCACAGGGAGACATTCACTATACC NGG 10 8 17 8 24 0 100 5 10
    240605014820_p0v7 ATATATCCAGTCAAGGAGCAATCACACTTTGG(+ACTCAC)GATCCGCATCTCAAAGATAGGGTGAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605014819_dw4d CATCTCCTCTCTCACCCTATCTTTGAGATGCG(+ACTCAC)GATCCCAAAGTGTGATTGCTCCTTGACTGGA NGG 10 8 17 8 24 0 100 5 10
    240605014816_9w5o AAAGGAAAGGTGTCTCTCCTACGGGCCAGGGG(+ACTCAC)GATCCTATCACAAAAGAATAAAGCAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605014815_qfrx TCCAATCAGGCTGCTTTATTCTTTTGTGATAG(+ACTCAC)GATCCCCCTGGCCCGTAGGAGAGACACCTTT NGG 10 8 17 8 24 0 100 5 10
    240605014814_g6tl AGGATCCCAGATCTCTTGGCCCATCCCATGAG(+ACTCAC)GTACCTGACTGTCTACCAGCACTGTCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605014813_t70n GGCTACCAGAGACAGTGCTGGTAGACAGTCAG(+ACTCAC)GTACCTCATGGGATGGGCCAAGAGATCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605014811_mggn CCCTGAGTGGGGTGGGTGGGGCTAGGAAGGAG(+ACTCAC)GTACCCCACAGCTTTCTGCATCTATATGGAG NGG 10 8 17 8 24 0 100 5 10
    240605014809_rq99 CTGGCCTCCATATAGATGCAGAAAGCTGTGGG(+ACTCAC)GTACCTCCTTCCTAGCCCCACCCACCCCACT NGG 10 8 17 8 24 0 100 5 10