Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014749_uvha CGATGGAGCCTGATGACATTAGAGCCCTTTTG(+ACTCAC)GTACCAGAAGGCAGCTGGAGCAGGGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605014747_mkt3 TGGCTCCCAGGCCCTGCTCCAGCTGCCTTCTG(+ACTCAC)GTACCAAAAGGGCTCTAATGTCATCAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605014746_g3nw ACTGGAAGGGAAAAGAAGGGATCTGGGTGCCG(+ACTCAC)GATCCTCAGGCGCAGGTGGTGACCACAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605014744_5lf5 CCTGGCCCCTGTGGTCACCACCTGCGCCTGAG(+ACTCAC)GATCCGGCACCCAGATCCCTTCTTTTCCCTT NGG 10 8 17 8 24 0 100 5 10
    240605014743_nzua CCCCAGAAACAAATGCAACCTACAATTGTGTG(+ACTCAC)GTACCGGGACCTCGAGTTCTGATCTGGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605014741_raay CTGACAAGACCAGATCAGAACTCGAGGTCCCG(+ACTCAC)GTACCACACAATTGTAGGTTGCATTTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605014739_tkzq ACAGTATCTAAAGGTAGTTATTTCTCCTTCTG(+ACTCAC)GATCCGGTGCAGCATACCTATGATTCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605014738_hcp7 TCTAGAGCTGGAATCATAGGTATGCTGCACCG(+ACTCAC)GATCCAGAAGGAGAAATAACTACCTTTAGAT NGG 10 8 17 8 24 0 100 5 10
    240605014736_665h TTTGATAGTAAGATTATAAGGTGGCTCCAGAG(+ACTCAC)GTACCTTGGATAGGACTGCGCCCTGATAAGA NGG 10 8 17 8 24 0 100 5 10
    240605014734_eps2 TTTCATCTTATCAGGGCGCAGTCCTATCCAAG(+ACTCAC)GTACCTCTGGAGCCACCTTATAATCTTACTA NGG 10 8 17 8 24 0 100 5 10