Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015322_qc0g TGCAGTTGCATGGGTAAAGCCACACCCAGAAG(+ACTCAC)GATCCCTGTTTGGACATCAGATGTTGTACTT NGG 10 8 17 8 24 0 100 5 10
    240605015320_op5d AAGGGTAGCAACAGCTTGTCTTCTGATTGATG(+ACTCAC)GTACCATCCCCCAAGAAGGGATCCTCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605015319_9nin TTCTTCCTGAGAGGATCCCTTCTTGGGGGATG(+ACTCAC)GTACCATCAATCAGAAGACAAGCTGTTGCTA NGG 10 8 17 8 24 0 100 5 10
    240605015318_g9j6 GGAGCTATGGAAGCATTGGTCTCTACACAGCG(+ACTCAC)GTACCCACAGGACCCACACAATGAGGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240605015316_1ntn AAATGCTAACCCTCATTGTGTGGGTCCTGTGG(+ACTCAC)GTACCGCTGTGTAGAGACCAATGCTTCCATA NGG 10 8 17 8 24 0 100 5 10
    240605015315_gfd3 TAGATGATCAACTTGATCCTGATTCAGCTTTG(+ACTCAC)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605015313_kvsk ACGAAATGGACAAATTTCTGGACAGATACCAG(+ACTCAC)GTACCAAAGCTGAATCAGGATCAAGTTGATC NGG 10 8 17 8 24 0 100 5 10
    240605015311_kjfr CTCTGCAGGCAAGCTCTCTTCTTGCAGGGCAG(+ACTCAC)GTACCCAGATATCTGGTGTTCGAACCAGACT NGG 10 8 17 8 24 0 100 5 10
    240605015310_hezx CCAGGAGTCTGGTTCGAACACCAGATATCTGG(+ACTCAC)GTACCTGCCCTGCAAGAAGAGAGCTTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605015308_2eis CTTTGGGCTGTCCTCCGTGAGTGTCATCCATG(+ACTCAC)GTACCTGCTCTAGGAATTCATTTCTCCAGAC NGG 10 8 17 8 24 0 100 5 10