Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015010_7hj4 AGGGGAGGGTTAGTTCTATCACCTTTTTTATG(+ACTCAC)GTACCTGATTGCTTTGCTTATCCATCAACAG NGG 10 8 17 8 24 0 100 5 10
    240605015009_8zm4 GAGCACTGTTGATGGATAAGCAAAGCAATCAG(+ACTCAC)GTACCATAAAAAAGGTGATAGAACTAACCCT NGG 10 8 17 8 24 0 100 5 10
    240605015008_k5gn GTTGCCTGCTTCTGAGAAAGTTATGTGTGTAG(+ACTCAC)GTACCATGTAAAGGATTTGGCTACAGATGTA NGG 10 8 17 8 24 0 100 5 10
    240605015007_nnp0 ACCAATACATCTGTAGCCAAATCCTTTACATG(+ACTCAC)GTACCTACACACATAACTTTCTCAGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605015005_h7vw TTCAGGATTTTCAGTCTTGCTGGCAATAGACG(+ACTCAC)GATCCGAAACCCTAAGTACAGGGATCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240605015004_fk4h CCTTCTTTCAGATCCCTGTACTTAGGGTTTCG(+ACTCAC)GATCCGTCTATTGCCAGCAAGACTGAAAATC NGG 10 8 17 8 24 0 100 5 10
    240605015003_8pua AAAAAAAATCAATCAAAATAACCTAATAAGTG(+ACTCAC)GATCCTATAATAATAAGCACCAGGCATGCCC NGG 10 8 17 8 24 0 100 5 10
    240605015002_pcms AGGGAGGGCATGCCTGGTGCTTATTATTATAG(+ACTCAC)GATCCACTTATTAGGTTATTTTGATTGATTT NGG 10 8 17 8 24 0 100 5 10
    240605015000_f997 TGTCATTACGGAGGTGCTGAAAATCAAACACG(+ACTCAC)GATCCTCCGGAAGAGCAGCTAGTGCTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605014958_qltc AGTGGTTAAAAGCACTAGCTGCTCTTCCGGAG(+ACTCAC)GATCCGTGTTTGATTTTCAGCACCTCCGTAA NGG 10 8 17 8 24 0 100 5 10