Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015151_koh6 TGACACTGAAGGAATAGGCAGAGGTTTTGAAG(+ACTCAC)GTACCTCATAGCTACCACAATGATAATAACA NGG 10 8 17 8 24 0 100 5 10
    240605015150_knvf GTGGTTGTTATTATCATTGTGGTAGCTATGAG(+ACTCAC)GTACCTTCAAAACCTCTGCCTATTCCTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605015148_1jgp CACACATCTGTGCACTGACTTTGCCATTGAGG(+ACTCAC)GATCCTCTGGGTTGGGTCTTTAAGACGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605015146_tpjp GTTTCCCTTCGTCTTAAAGACCCAACCCAGAG(+ACTCAC)GATCCCTCAATGGCAAAGTCAGTGCACAGAT NGG 10 8 17 8 24 0 100 5 10
    240605015145_k2bb GCCCTTCCCACCTTTGCCTGTCTACAGTAGTG(+ACTCAC)GATCCATTTCCTCCCGAGGTTGCCCATCAAT NGG 10 8 17 8 24 0 100 5 10
    240605015144_08bl ATCCAATTGATGGGCAACCTCGGGAGGAAATG(+ACTCAC)GATCCACTACTGTAGACAGGCAAAGGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605015143_h20q ATACTCCCAGAATGAGCAGAGGGTCCAAAGTG(+ACTCAC)GATCCCTGAGCCATTTTGAATAATTTTATCA NGG 10 8 17 8 24 0 100 5 10
    240605015141_p2pw AGTGGTGATAAAATTATTCAAAATGGCTCAGG(+ACTCAC)GATCCACTTTGGACCCTCTGCTCATTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605015140_jeoj TAGCACAGGGTCCAGGCAACCTCAGCCTATGG(+ACTCAC)GATCCTTAGGCACTGTGTCACAGGAACGTCC NGG 10 8 17 8 24 0 100 5 10
    240605015138_oyqi AGCTAGGACGTTCCTGTGACACAGTGCCTAAG(+ACTCAC)GATCCCATAGGCTGAGGTTGCCTGGACCCTG NGG 10 8 17 8 24 0 100 5 10