Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014213_1fg8 GGCGTGGCAGACTGCTGAAGGACCTGGCTGAG(+ACTCAC)GTACCATCACACCAGTCACAACAGGAGGATA NGG 10 8 17 8 24 0 100 5 10
    240605014212_nspy TCAAGTCCTTGGGGTTTTGTTATTTTGCACTG(+ACTCAC)GATCCACCTGTTGTAAATAGTCTTAAATACA NGG 10 8 17 8 24 0 100 5 10
    240605014211_5bcg AAATGTGTATTTAAGACTATTTACAACAGGTG(+ACTCAC)GATCCAGTGCAAAATAACAAAACCCCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605014208_15rr CCAAGTGCAGAGCCAGTGTTGGACCACCTCAG(+ACTCAC)GATCCCCTTGTGGAGACTGCCTGGTGACCTC NGG 10 8 17 8 24 0 100 5 10
    240605014207_krwt ATTCAGAGGTCACCAGGCAGTCTCCACAAGGG(+ACTCAC)GATCCTGAGGTGGTCCAACACTGGCTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605014205_uhz2 GCCGCAGGCGCCCAAGCGGACTCACCCGGCAG(+ACTCAC)GATCCGAGTCCTAGGCGCGGCACAGTGGCGG NGG 10 8 17 8 24 0 100 5 10
    240605014204_f3gn CACCGCCGCCACTGTGCCGCGCCTAGGACTCG(+ACTCAC)GATCCTGCCGGGTGAGTCCGCTTGGGCGCCT NGG 10 8 17 8 24 0 100 5 10
    240605014202_pzy8 TAAAATAATGAGACAGTTTTTTGTTATTGGTG(+ACTCAC)GATCCAGGAGCTGTTTAGAAACTATGTATTT NGG 10 8 17 8 24 0 100 5 10
    240605014200_aazm GGGGAAAATACATAGTTTCTAAACAGCTCCTG(+ACTCAC)GATCCACCAATAACAAAAAACTGTCTCATTA NGG 10 8 17 8 24 0 100 5 10
    240605014158_hyq6 TGGGGCCCAGCTGCGGCTGGCTCCCGGCCTGG(+ACTCAC)GTACCAGAAAGAGACCTTCAGCTCAACCCAG NGG 10 8 17 8 24 0 100 5 10