Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014127_sqv5 CCTACACCCAACCACTCTACACTGAATAGATG(+ACTCAC)GATCCTCCATCAAAGAATTGTCTTTCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605014126_jis3 GACATTAAAAGAAAGACAATTCTTTGATGGAG(+ACTCAC)GATCCATCTATTCAGTGTAGAGTGGTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605014124_9h1b TTCGAAGGCTAACATGAGAGATGTTACAGATG(+ACTCAC)GTACCTCAAAGTTTTTCAAATTCACTTGACT NGG 10 8 17 8 24 0 100 5 10
    240605014123_47jy TGATCAGTCAAGTGAATTTGAAAAACTTTGAG(+ACTCAC)GTACCATCTGTAACATCTCTCATGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605014122_bn90 TTCCAAGGCTAACATGAGAGATGTTACAGATG(+ACTCAC)GTACCTCAAAGTTTTTCAAATTCACTTGACT NGG 10 8 17 8 24 0 100 5 10
    240605014121_1yby TGATCAGTCAAGTGAATTTGAAAAACTTTGAG(+ACTCAC)GTACCATCTGTAACATCTCTCATGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605014120_rvsh TTCCAAGGCTAACATGAGAGATGTTACAGATG(+ACTCAC)GTACCTCAAAGTTTTTCAAATTCACTTGACT NGG 10 8 17 8 24 0 100 5 10
    240605014118_l0ki TGATCAGTCAAGTGAATTTGAAAAACTTTGAG(+ACTCAC)GTACCATCTGTAACATCTCTCATGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605014117_hrb7 ATGTATGTTTTGCCTGCCACAACCCGTACCTG(+ACTCAC)GTACCAGCAAAACCTGCAAAAAGCAAACAAG NGG 10 8 17 8 24 0 100 5 10
    240605014116_q6j8 CCTGGCTTGTTTGCTTTTTGCAGGTTTTGCTG(+ACTCAC)GTACCAGGTACGGGTTGTGGCAGGCAAAACA NGG 10 8 17 8 24 0 100 5 10