Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014100_tfgd AATTTCCTTTCTCCGTGAATTGTCACTAAGAG(+ACTCAC)GTACCAAACTTGCCTGTTTGAAGCCATCCTC NGG 10 8 17 8 24 0 100 5 10
    240605014058_gbui AAGAAGGAGGAGGAGGGATGAGTGGTCTAGTG(+ACTCAC)GTACCCTCGTGCCAACAGGGTTCGTGGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605014056_vg6g TTTGCACCACCACGAACCCTGTTGGCACGAGG(+ACTCAC)GTACCACTAGACCACTCATCCCTCCTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605014055_3vbc TCATAAAAAATAACTCATAGGTACAAGTAATG(+ACTCAC)GTACCATATGAGTATGAGTGTAGCCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605014054_hnba GCTCAGAGAAAGGCTACACTCATACTCATATG(+ACTCAC)GTACCATTACTTGTACCTATGAGTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240605014053_agds CAGAAAATGTTCTTAGGGCATCATGTCACAAG(+ACTCAC)GATCCTTGTAACATATTGGCTCAAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605014052_kxsn AGTCTAGAAAATTTGAGCCAATATGTTACAAG(+ACTCAC)GATCCTTGTGACATGATGCCCTAAGAACATT NGG 10 8 17 8 24 0 100 5 10
    240605014051_cazl GGTGAGTACCACTATGCTTGGCTTCCCTTTTG(+ACTCAC)GATCCTCTCCCCCTTTCAGCAAAAAAAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605014049_53pd AGGGTAACTTTTTTTTGCTGAAAGGGGGAGAG(+ACTCAC)GATCCAAAAGGGAAGCCAAGCATAGTGGTAC NGG 10 8 17 8 24 0 100 5 10
    240605014047_lu1w GCTGCATGAATGAAATGATGTGATGATGGAGG(+ACTCAC)GATCCTGCTTTCTAAACTTTATGAACTCTCA NGG 10 8 17 8 24 0 100 5 10