Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014003_mstx AATGTATGTTATGGAAACTTGAAAGTATGATG(+ACTCAC)GATCCATAAGTTAATAAGATTCAAAACTCAA NGG 10 8 17 8 24 0 100 5 10
    240605014002_cndp CAGTTTAGTTACCCCACAAAGGGCAATATATG(+ACTCAC)GATCCTTAGGTTAGCCTTGTTTTATGAAACA NGG 10 8 17 8 24 0 100 5 10
    240605014000_23yi AGTCCTGTTTCATAAAACAAGGCTAACCTAAG(+ACTCAC)GATCCATATATTGCCCTTTGTGGGGTAACTA NGG 10 8 17 8 24 0 100 5 10
    240605013959_k8ba TCACATGTACATGGGCACTGGCAGGCACAGAG(+ACTCAC)GTACCTATTGCATGCACACCAAGCAACAAGT NGG 10 8 17 8 24 0 100 5 10
    240605013958_zqi9 TGCACACTTGTTGCTTGGTGTGCATGCAATAG(+ACTCAC)GTACCTCTGTGCCTGCCAGTGCCCATGTACA NGG 10 8 17 8 24 0 100 5 10
    240605013956_zvb8 TGCTCCTGTGTAATCATTATCCTTTGTTGTAG(+ACTCAC)GATCCTTAAGAAGATAATCAAAATATCCTAA NGG 10 8 17 8 24 0 100 5 10
    240605013955_xjk6 TTCCTTTAGGATATTTTGATTATCTTCTTAAG(+ACTCAC)GATCCTACAACAAAGGATAATGATTACACAG NGG 10 8 17 8 24 0 100 5 10
    240605013954_q946 CCTATGATCAAATTCTGGAGTCTTTGTTCTCG(+ACTCAC)GTACCTCCTAAAAAGGAAAGTTGATTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605013953_8o2r GTTACACAAGAATCAACTTTCCTTTTTAGGAG(+ACTCAC)GTACCGAGAACAAAGACTCCAGAATTTGATC NGG 10 8 17 8 24 0 100 5 10
    240605013951_znhg GTTCTAGATGCTTGAGATCAGTAAGCTAAGCG(+ACTCAC)GTACCCAAGGGGGCATCCCCCTCTCTGCGGA NGG 10 8 17 8 24 0 100 5 10