Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241120174827_venv GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCA(/T)GCACGCTGCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGCTCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10
    241120174634_6fn2 GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAA(/T)GCACGCTGCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGCTCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10
    241120151034_bsxk CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241119223448_mvwu CCAGAACCGGAGGACAAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCG(-AGC)AGAAGAAGAAGGGCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAGGCCAA NGG 10 8 17 8 24 0 100 5 10
    241119222318_8aep AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGTGC(/A)CAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCAGCTTCCTCCTCCTTTATTTT NGG 10 8 17 8 24 0 100 5 10
    241119221846_tv9b TGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCT(/A)GTGCCAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCAGCTTCCTCCTCCTTTA NGG 10 8 17 8 24 0 100 5 10
    241119221726_oc7t TGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTG(/A)GTGCCAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCAGCTTCCTCCTCCTTTA NGG 10 8 17 8 24 0 100 5 10
    241119220205_1drc AGGATAAGGCCAACCATGATGTGCTGAAACTGGAACA(+TAG)CAACCACCCACAAGTCATTGGGGTAGAAGCGGTCACAGATATATCTGTCATCTGCCTCACTGA NGG 10 8 17 8 24 0 100 5 10
    241119214416_5pgv AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGA(+C)TTCCTGGTGCCAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241119213932_x6l0 AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGA(+CAC)TTCCTGGTGCCAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10