Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241122093610_zy2t CATGGGAACTCAGTTTATATGAGTTACAACGAACACCTCAGGTAATGACTAAGATGACTGC(-C)AAGGGGCATATGAGACGTGTAAACTGGGATACATTTTT NGG 10 8 17 8 24 0 100 5 10
    241122085307_s19j TTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGGCCCAGACTGAGCACGTGATGGCAGAGGAAAGGAA(-G)CCCTGCTTCCTCCAGAGGGCGTCGCAGGACAG NGG 10 8 17 8 24 0 100 5 10
    241122085239_fvsf TTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGGCCCAGACTGAGCACGTGATGGCAGAGGAAAGGAA(-G)CCCTGCTTCCTCCAGAGGGCGTCGCAGGACAG NGG 10 8 17 8 24 0 100 5 10
    241122002859_pw0n TTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGGCCCAGACTGAGCACGTGATGGCAGA(/C)GGAAAGGAAGCCCTGCTTCCTCCAGAGGGCGTCGCAGGACAG NGG 10 8 17 8 24 0 100 5 10
    241122002251_3obb TTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGGCCCAGACTGAGCACGTGATGGCAGA(/G)GGAAAGGAAGCCCTGCTTCCTCCAGAGGGCGTCGCAGGACAG NGG 10 8 17 8 24 0 100 5 10
    241122000624_sa9l TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGG(-TGC)TGCAGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241121234221_qmjb TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATTCCA(-TGA)GGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241121234211_3prr TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATTCCA(-TGA)GGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241121182340_oszq CTCCTCTGCTTGACCCCGGGTGTCCACGAATGGTGTCCTGACCCTCTTGGGACGCTGAATGCCTGGAGCTGTCTCG(/A)TGCCTGCTCGTGGTGCGATCCTTGTCTTCCTCCAGTGCTGGTCCCGGTCCGTCCATGGGCAGAGTCAGGCTGTT NGG 10 8 17 8 24 0 100 5 10
    241121170633_9j52 TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGC(+ACT)AGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10