Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241122143206_cozb AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCT(/A)GGTGACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122143155_qoio AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCT(/C)GGTGACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122143136_frm0 AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGG(/C)TGACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122143104_03k1 AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTG(/C)GTGACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122143016_ql4a AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTG(/C)GTGACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122142914_7pg7 AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGTGCCAGAA(/G)ACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122142855_jtd4 AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGTGCCAGAAA(/T)CAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122142844_fqoo AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGTGCCAGAAA(/C)CAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122142831_2wgq AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGTGCCAGAAA(/G)CAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122142443_zx95 AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGA(+C)TTCCTGGTGCCAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10