Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250303045232_ossh CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250302065514_cx3b CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250302065420_0ch3 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250301172840_iaee GCGAAGCCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATCC(/G)ATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAACACTGGTCGGAGGCG NGG 10 8 17 8 24 0 100 5 10
    250228182226_eodl CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250227211449_0thu CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250227111159_bwgu GAGTGTGTGCGTAAAGGGGGCTCTCCAGCAGTGTGTAAAGCATCTCCAGCAGAGTGTAAAGCCTCTCCGGTGTGTGTAAAGCCTCTCCGGTGTGTGTAAAGCCTCTCCGGTGTGTGTGAAGCGTCTCCGGTGTGTGTGAAGCCTCTCACTCTCCTCGACTCTTCATCATC(+GCCACCATGGC)ATGTCTCTCCATCTCCTCCTCTTCTTCTTCCTCCAGCTCTTCAGCTCGTCTCTCCAGCAGTTCCCCCGAGTCTGCACCTCCCCAGAAGTCCTCCAGTCCA NG 10 8 17 8 24 0 100 5 10
    250227110031_5tuw AACTGTAATATGTCTGTATTTATCTTTCAGGTAGCTGATTAACAGCTGTACCAGAGGCAAACAGACACACCTGCAGTCTCTCTTTCTGCTGTCAGACACACCTGAGAAACAGACAGACTGACAGAG(+GCCACCATGGC)ATGTACACAGCACTTCTGCTCTCCTCCTCTCTGTTTCTCCTGCATGTGACCTGCACACCTCAGACTCACAGTCATCACGGGTAAAAACTCACATAAACACACACACACACACACACACTGTTGTCTGTAATTTCAGTTCACATGCTCTGGGGCATCAGGGGTCACAGGAAGGTTAAAGGTCAGTGCATATAATAACAGCTAATTACATTAGTCAGTCACGTCCATTTAGAACTG NG 10 8 17 8 24 0 100 5 10
    250225172035_u703 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250225171212_s52m CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10