Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241122150757_bjgt TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATT(-CCA)TGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122150655_zcoi TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATT(-CCA)TGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122150635_bo1j TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATTCC(-ATG)AGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122150634_zkzq TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATTCC(-ATG)AGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122150612_fa43 TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATTCCATG(-AGG)TGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122150550_xqoc TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGC(-TGC)AGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122150529_fx8u TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGG(-TGC)TGCAGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122145730_68zk TAGTGCTTGAGACCGCCAGAAGCTCGGAAAAGCG(+ACT)ATCCAGGTGCTGCAGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTG NGG 10 8 17 8 24 0 100 5 10
    241122145632_qjvv TAGTGCTTGAGACCGCCAGAAGCTCGGAAAAGCG(+ACT)ATCCAGGTGCTGCAGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTG NGG 10 8 17 8 24 0 100 5 10
    241122145521_nqkg TAGTGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGT(+ACT)GCTGCAGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTG NGG 10 8 17 8 24 0 100 5 10