Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241124131006_6wo7 GATAAAGACTGTGATGTTTGACAAGACTGGCACCATTACCCATGGCGTCCCCAGGGTCATGCGGGTGCTCCTGCTGGGGGATGTGGCCACACTGCCCCTCAGGAAGGTTCTGGCTGTGGTGGGGACTGCGGAGGCCAGCAGTGAACAA(/C)CCCTTGGGCGTGGCAGTCACCAAATACTGTAAAGAGG NGG 10 8 17 8 24 0 100 5 10
    241123012850_a323 agacaaggtggagagagtgaaacattcgccaaaagagcaattgaaagtttagttaaaaagctgaaggagaaaaaagatgaattggattctttaataacagctataactacaaatggagctcaccctagcaagtgtgttac(/T)catacagagaacattggatggaaggcttcaggttagtcttgttccagggtctcttctgtactctgtagtggcaggtttctacagattcttgtttcctctcaagttactacggaagttttatgcttgctccgtctctttagatactgtagtacatcctatagcacagatatt NGG 10 8 17 8 24 0 100 5 10
    241123011647_203d CGCCGTCGGGCCCTCGCCACCATGTACGTGAGCTACCTCCTGGACAAGGACGTGAGCATGTATCCCAGCTCCGTGCGCCACTCCGGCAGCCTCAACCTGGCCCCGCAGAACTTCGTCAGCCCCCCGCAGTACCCGGAC(/T)TACGGCGGATACCATGTGGCGGCCGCGGCCGCCGCCGCCGCCAACCTGGACAGCGCGCAGTCCCCGGGGCCGTCCTGGCCTGCCGCCTACGGCGCCCCGCTCCGCGAGGACTGGAACGGCTACACGCCGGGGGGCGCGGCGGCCGCCGCCAACGCCGTAGCGCACGGCCTCAACGGGGGCTCCCCGGCCGC NGG 10 8 17 8 24 0 100 5 10
    241123010705_km38 GGGAGGGCGGAACCGAGGGCCCGTCGGTGATGGATCATTCACTCATTCATTCTTTCAGTCTGTGATGTGGATGGGGGGCTGTGGCGGATGGGGGGTTGGGGGGAACCCCGTGGGGAGCGGCCTTCGGGGAGAAGGCAGGAACAGGAGTCCCTTTTCCTGCCTCAGGTGTCCAGCCACATC(/T)CAGGTGCTGGCTCGGCGAAAAGCCCGTGAGATCCAGGCCAAGCTAAAGGTAAGACAGGGGTGGCGGTGGCAGCACAGGCCCAGGGCTCGGGAGGGCTGGCCTCTGGGACTTTGGGCTCATTTCCCAGGAGGAAAAGTCTTTCTGTGGGTTTCGGGACTGCCTGCAGAGCCAGCGGGCTTCAAGGTGCTGAGGAAAGTGGGGTTCAGCCTGTAACC NGG 10 8 17 8 24 0 100 5 10
    241123003439_oktu AGACAAGGTGGAGAGAGTGAAACATTCGCCAAAAGAGCAATTGAAAGTTTAGTTAAAAAGCTGAAGGAGAAAAAAGATGAATTGGATTCTTTAATAACAGCTATAACTACAAATGGAGCTCACCCTAGCAAGTGTGTTAC(/T)CATACAGAGAACATTGGATGGAAGGCTTCAGGTTAGTCTTGTTCCAGGGTCTCTTCTGTACTCTGTAGTGGCAGGTTTCTACAGATTCTTGTTTCCTCTCAAGTTACTACGGAAGTTTTATGCTTGCTCCGTCTCTTTAGATACTGTAGTACATCCTATAGCACAGATATT NGG 10 8 17 8 24 0 100 5 10
    241123000422_j8su CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241122160741_q2hu CATGGGAACTCAGTTTATATGAGTTACAACGAACACCTCAGGTAATGACTAAGATGACT(/A)GCCAAGGGGCATATGAGACGTGTAAACTGGGATACATTTTT NGG 10 8 17 8 24 0 100 5 10
    241122160522_jbtn CATGGGAACTCAGTTTATATGAGTTACAACGAACACCTCAGGTAATGACTAAGATGACT(/A)GCCAAGGGGCATATGAGACGTGTAAACTGGGATACATTTTT NGG 10 8 17 8 24 0 100 5 10
    241122154122_ugb5 AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCT(/A)GGTGCCAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122154029_7bpg AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCT(/A)GGTGCCAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10