Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241203234440_rz3i TTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATTCCATGAGGTGC(/A)GCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACGGCG NGG 10 8 17 8 24 0 100 5 10
    241203234409_a39c TTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGC(/A)TGCAGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACGGCG NGG 10 8 17 8 24 0 100 5 10
    241203234346_x5q0 TTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGG(/T)TGCTGCAGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACGGCG NGG 10 8 17 8 24 0 100 5 10
    241203233947_hpso TTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAG(/T)GGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACGGCG NGG 10 8 17 8 24 0 100 5 10
    241203233930_5n8x TTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGA(/C)AGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACGGCG NGG 10 8 17 8 24 0 100 5 10
    241203233912_hjw5 TTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATTCCATGAGGT(/A)GCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACGGCG NGG 10 8 17 8 24 0 100 5 10
    241203233818_yuog TTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATTCCATGAGG(/C)TGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACGGCG NGG 10 8 17 8 24 0 100 5 10
    241203233757_2b4o TTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATTCCATGAGGTGC(/A)GCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACGGCG NGG 10 8 17 8 24 0 100 5 10
    241203233636_elvu TTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATTCCATGAGGT(/C)GCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACGGCG NGG 10 8 17 8 24 0 100 5 10
    241202204637_81n3 GAACGGGGACCTGCCCTGGCCACCGCTCAGGAATGAATTCAGATATT(/C)TCCAGAGAATGACCACAACCTCTTCAGTAGAAG NGG 10 8 17 8 24 0 100 5 10