Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241202125959_kvby GAACGGGGACCTGCCCTGGCCACCGCTCAGGAATGAATTCAGATATTT(/C)CCAGAGAATGACCACAACCTCTTCAGTAGAAG NGG 10 8 17 8 24 0 100 5 10
    241202084838_rb7g GAACGGGGACCTGCCCTGGCCACCGCTCAGGAATGAATTCAGATATT(/C)TCCAGAGAATGACCACAACCTCTTCAGTAGAAG NGG 10 8 17 8 24 0 100 5 10
    241201124015_2cf6 GATAAAGACTGTGATGTTTGACAAGACTGGCACCATTACCCATGGCGTCCCCAGGGTCATGCGGGTGCTCCTGCTGGGGGATGTGGCCACACTGCCCCTCAGGAAGGTTCTGGCTGTGGTGGGGACTGCGGAGGCCAGCAGTGAACAA(/C)CCCTTGGGCGTGGCAGTCACCAAATACTGTAAAGAGG NGG 10 10 17 10 24 0 100 5 20
    241201122743_p9sz CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 10 17 10 24 0 100 5 10
    241201122616_uln9 GATAAAGACTGTGATGTTTGACAAGACTGGCACCATTACCCATGGCGTCCCCAGGGTCATGCGGGTGCTCCTGCTGGGGGATGTGGCCACACTGCCCCTCAGGAAGGTTCTGGCTGTGGTGGGGACTGCGGAGGCCAGCAGTGAACAA(/T)CCCTTGGGCGTGGCAGTCACCAAATACTGTAAAGAGG NGG 10 12 17 10 24 0 100 5 10
    241201081713_ix6r TAGGAGACGCTCTGTCTCTTCCTTCCCAGTCCCCCAGGACAATGTGGATA(/G)CTCATCCTGGTTCTGGTAAAGAAGTGAGGGTACCAGCTACTGCC NGG 10 8 17 8 24 0 100 5 10
    241201081326_tov9 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241129192636_48e4 ACCTGAAGATGAGAGCGGCAGAGACTATAGGGAGAGGGAACGGGAATATGAACGAGATCAGGAGCG(/A)CATACTTCGAGAAAGAGAGAGGCTGAAGCGGCAAGAAGAAGAGCGCCGTAGGCAGAAGGAGCGCTATGAGAAAGAGAAGACTTTTAAGAGAAAAGAAGAAGAAATGAAAAAAGAGAAAGACACACTTCGGGATAAAGGAAAGAAGGCTGAAAGTACAGAATCAATAGGCAGCTCAGAAAAAACTGAAAAGAAAGAAGAAGTGGTCAAGAGAGATCGAATAAGAAACAAG NGG 10 8 17 8 24 0 100 5 10
    241128124856_cabk CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241127171542_bgu5 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10