Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241122153619_7ieg AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGTGCCAGA(/C)AACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122151645_p5rb TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGA(-TTC)CATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122151619_m6ep TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGA(-TTC)CATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122151556_v6vn TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGAT(-TCC)ATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122151534_xgxi TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGA(-TTC)CATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122151508_0uji TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGG(-ATT)CCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122151448_1snv TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGG(-GAT)TCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122151409_43ht TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGA(-TTC)CATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122151036_gtnw TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGGATT(-CCA)TGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10
    241122151008_bs1n TGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGCAGAAGGG(-ATT)CCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACG NGG 10 8 17 8 24 0 100 5 10