Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241122145456_l3h6 TAGTGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAGGTGCTGC(+ACT)AGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTG NGG 10 8 17 8 24 0 100 5 10
    241122144855_lx45 AAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCG(/A)AGCAGAAGAAGAAGGGCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    241122144348_akvd AAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCCCATCA(-CAT)CAACCGGTGGCGCATTGCCACGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    241122143845_3rkd AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTG(-G)TGACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122143810_3933 AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCC(-T)GGTGACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122143753_4fdg AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTC(-C)TGGTGACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122143735_6efz AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATT(-C)CTGGTGACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122143257_uh3k AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGT(/G)GACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122143245_4gz8 AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGT(/C)GACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10
    241122143233_27yy AACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGT(/A)GACAGAAACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCA NGG 10 8 17 8 24 0 100 5 10