Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250407222231_hjji TCCTCGGCCCTGCACCACACCGGACGTCCTGCAGAGCAAGCGCTGCTGTCCAGTTTGGCCGGGCGACGGTTCGGTGTGCGGATCCCTTTCGGGTCGAGGCTTCTGCCAGGATGTCACGGTCTCCGACCTTCCCAAC(-GG)GCCGCAGTTCCCTCATTCAGACGTGGACGACCGCGAACGCTGGCCTCTGGTGTTTTACAACCAAACCTGCCAGTGCGCCGGCAACTACATGGGGTTCAACTGCGGCGAGTGCAAGTTCGGTTACTTCGGCGCCAACTGCGGGGAAAGACGGGAGTCTGTTCGCAGAAACATCTTCCAGTTGTCCATGTCCGAACAGCAGCGGTTCATCTCATACCTAAATCTCGC NGG 10 8 17 8 24 0 100 5 10
    250407221648_u7v8 GATCCGGAAGCTGACCGGTGACTTTAACTTTACTATCCCGTACTGGGACTGGCGCGACGCGCAGGACTGTCAGGTGTGCACGGACGAGCTGATGGGGGCCCGCAGTCCTCTCAGCCCCAAC(+TGA)CTGATCAGCCCGTCCTCGGTGTTCTCCTCCTGGAAGGTAAACATGAAAACAAATTCATTTTACTCAAGAATACAGTATTTATCCCTCTAGTCATGTTGGTTCTATTCATTTGGAATTCATTCCAATTACACAAATGCTTGTTTTCTCTTGCAGACACACAAAACCAAAC NGG 10 8 17 8 24 0 100 5 10
    250407221534_ph8a TCCTCGGCCCTGCACCACACCGGACGTCCTGCAGAGCAAGCGCTGCTGTCCAGTTTGGCCGGGCGACGGTTCGGTGTGCGGATCCCTTTCGGGTCGAGGCTTCTGCCAGGATGTCACGGTCTCCGACCTTCCCAAC(+TGA)GGGCCGCAGTTCCCTCATTCAGACGTGGACGACCGCGAACGCTGGCCTCTGGTGTTTTACAACCAAACCTGCCAGTGCGCCGGCAACTACATGGGGTTCAACTGCGGCGAGTGCAAGTTCGGTTACTTCGGCGCCAACTGCGGGGAAAGACGGGAGTCTGTTCGCAGAAACATCTTCCAGTTGTCCATGTCCGAACAGCAGCGGTTCATCTCATACCTAAATCTCGCG NGG 10 8 17 8 24 0 100 5 10
    250407221219_ig2u TCCTCGGCCCTGCACCACACCGGACGTCCTGCAGAGCAAGCGCTGCTGTCCAGTTTGGCCGGGCGACGGTTCGGTGTGCGGATCCCTTTCGGGTCGAGGCTTCTGCCAGGATGTCACGGTCTCCGACCTTCCCAAC(+TGA)GGGCCGCAGTTCCCTCATTCAGACGTGGACGACCGCGAACGCTGGCCTCTGGTGTTTTACAACCAAACCTGCCAGTGCGCCGGCAACTACATGGGGTTCAACTGCGGCGAGTGCAAGTTCGGTTACTTCGGCGCCAACTGCGGGGAAAGACGGGAGTCTGTTCGCAGAAACATCTTCCAGTTGTCCATGTCCGAACAGCAGCGGTTCATCTCATACCTAAATCTCGCG NGG 10 8 17 8 24 0 100 5 10
    250407221047_tbbs AAACATCTTCCAGTTGTCCATGTCCGAACAGCAGCGGTTCATCTCATACCTAAATCTCGCGAAGACCACCATCAGCCCCGATTACATGATCGTGACTGGCACGTACGCGCAGATGAACAATGGCTCGACGCCCATG(+TGA)TTCGCAAACATCAGCGTGTACGACCTGTTCGTGTGGATGCACTATTACGTGTCCCGTGACGCGCTGCTCGGGGGTCCCGGGAACGTGTGGGCCGACATTGATTTTGCGCACGAGTCGGCCGCTTTCCTGCCCTGGCACCGAGTTTACCTGCTGTTCTGGGAGCACGA NGG 10 8 17 8 24 0 100 5 10
    250407220532_p57c GATCCGGAAGCTGACCGGTGACTTTAACTTTACTATCCCGTACTGGGACTGGCGCGACGCGCAGGACTGTCAGGTGTGCACGGACGAGCTGATGGGGGCCCGCAGTCCTCTCAGCCCCAAC(+TGA)CTGATCAGCCCGTCCTCGGTGTTCTCCTCCTGGAAGGTAAACATGAAAACAAATTCATTTTACTCAAGAATACAGTATTTATCCCTCTAGTCATGTTGGTTCTATTCATTTGGAATTCATTCCAATTACACAAATGCTTGTTTTCTCTTGCAGACACACAAAACCAAAC NGG 10 8 17 8 24 0 100 5 10
    250407220423_7ftv GATCCGGAAGCTGACCGGTGACTTTAACTTTACTATCCCGTACTGGGACTGGCGCGACGCGCAGGACTGTCAGGTGTGCACGGACGAGCTGATGGGGGCCCGCAGTCCTCTCAGCCCCAA(+CTGA)CCTGATCAGCCCGTCCTCGGTGTTCTCCTCCTGGAAGGTAAACATGAAAACAAATTCATTTTACTCAAGAATACAGTATTTATCCCTCTAGTCATGTTGGTTCTATTCATTTGGAATTCATTCCAATTACACAAATGCTTGTTTTCTCTTGCAGACACACAAAACCAAAC NGG 10 8 17 8 24 0 100 5 10
    250407220101_52w9 GATCCGGAAGCTGACCGGTGACTTTAACTTTACTATCCCGTACTGGGACTGGCGCGACGCGCAGGACTGTCAGGTGTGCACGGACGAGCTGATGGGGGCCCGCAGTCCTCTCAGCCCCAAC(+TGA)CTGATCAGCCCGTCCTCGGTGTTCTCCTCCTGGAAGGTAAACATGAAAACAAATTCATTTTACTCAAGAATACAGTATTTATCCCTCTAGTCATGTTGGTTCTATTCATTTGGAATTCATTCCAATTACACAAATGCTTGTTTTCTCTTGCAGACACACAAAACCAAAC NGG 10 8 17 8 24 0 100 5 10
    250407220013_9o9w GATCCGGAAGCTGACCGGTGACTTTAACTTTACTATCCCGTACTGGGACTGGCGCGACGCGCAGGACTGTCAGGTGTGCACGGACGAGCTGATGGGGGCCCGCAGTCCTCTCAGCCCC(+T)AACCTGATCAGCCCGTCCTCGGTGTTCTCCTCCTGGAAGGTAAACATGAAAACAAATTCATTTTACTCAAGAATACAGTATTTATCCCTCTAGTCATGTTGGTTCTATTCATTTGGAATTCATTCCAATTACACAAATGCTTGTTTTCTCTTGCAGACACACAAAACCAAAC NGG 10 8 17 8 24 0 100 5 10
    250406020959_n2vf TTATAATGAGAGCATTTCTTAAGGAAAAAAGACTTGGAAGTACAAGAtttttttGTTTTCTCATAGATGTTCTTGAGGGCTATAATGGAACAATATTTGCATATGGACAAACATCATCTGGGAA(/G)GACCCACACGATGGAGGTAATGTTTCATAATCTATCCTTAAGTATAGTTTACACTATAAGTCATCCACTTACTGATTTCTTGTAGTTAATTCATTGTGCTTAATTTTTTATCTTTAT NGG 10 8 17 8 24 0 100 5 10