Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013849_yu7t TCTTTTATGTGTGTCTAAATAAACACTGAATG(+ACTCAC)GTACCAACCTGGCTGTGGGGACATTTATGTC NGG 10 8 17 8 24 0 100 5 10
    240605013847_elc5 CCTGGGACATAAATGTCCCCACAGCCAGGTTG(+ACTCAC)GTACCATTCAGTGTTTATTTAGACACACATA NGG 10 8 17 8 24 0 100 5 10
    240605013846_pu3j ACAACCTACCTGCTCTGCTGTTTCTGGAGTTG(+ACTCAC)GTACCATACAGTTCCCTGGCATGGAAGGATC NGG 10 8 17 8 24 0 100 5 10
    240605013845_ojkl GGCCCGATCCTTCCATGCCAGGGAACTGTATG(+ACTCAC)GTACCAACTCCAGAAACAGCAGAGCAGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605013844_mggf CATTTAGGGTACACTGGCAAAGGAAGGGGCTG(+ACTCAC)GATCCTAGTTTGGCTAAAAGGCAGGCTGCAA NGG 10 8 17 8 24 0 100 5 10
    240605013842_2w0h ACTCATTGCAGCCTGCCTTTTAGCCAAACTAG(+ACTCAC)GATCCAGCCCCTTCCTTTGCCAGTGTACCCT NGG 10 8 17 8 24 0 100 5 10
    240605013841_3rzg AGTGTCAGGTCTGACTGCAGACGCTCTTTAGG(+ACTCAC)GTACCCACTCTTCACCCTCACATAACACTAC NGG 10 8 17 8 24 0 100 5 10
    240605013839_i590 TGTGTGTAGTGTTATGTGAGGGTGAAGAGTGG(+ACTCAC)GTACCCTAAAGAGCGTCTGCAGTCAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240605013838_1hqe GAACCTGGAATAGCTGTCGGGGCTTCAGCGAG(+ACTCAC)GATCCCTCCAAACCTCATGTTCTGTCCCGAT NGG 10 8 17 8 24 0 100 5 10
    240605013836_2q84 GCGACATCGGGACAGAACATGAGGTTTGGAGG(+ACTCAC)GATCCTCGCTGAAGCCCCGACAGCTATTCCA NGG 10 8 17 8 24 0 100 5 10