Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013745_4888 CATAACAACTGCAGGTGCTCAGACCACTGTTG(+ACTCAC)GTACCAGAACCAGTGCCTTGGTGGGGCCATC NGG 10 8 17 8 24 0 100 5 10
    240605013744_tarq GGCAAGATGGCCCCACCAAGGCACTGGTTCTG(+ACTCAC)GTACCAACAGTGGTCTGAGCACCTGCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605013742_ui1w TACTGTACTGGGTTCCGTGTGTCACTGGGGTG(+ACTCAC)GTACCTATATTTTAATACTGTTCCTAACATT NGG 10 8 17 8 24 0 100 5 10
    240605013741_nf5c AATGAAATGTTAGGAACAGTATTAAAATATAG(+ACTCAC)GTACCACCCCAGTGACACACGGAACCCAGTA NGG 10 8 17 8 24 0 100 5 10
    240605013740_74vc CTTCCATGAACTCTTTAGGCCATGATTCTGTG(+ACTCAC)GTACCTAGTTATGATAGTACAAATCAGATCA NGG 10 8 17 8 24 0 100 5 10
    240605013738_haj5 TTCTCTGATCTGATTTGTACTATCATAACTAG(+ACTCAC)GTACCACAGAATCATGGCCTAAAGAGTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605013736_dn7n AGTTTTTACACCATGGAAATGGGGTACACGGG(+ACTCAC)GTACCCAGGTCATCAGCATGCCCTCTAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605013734_ex2s TGGGTACTTTAGAGGGCATGCTGATGACCTGG(+ACTCAC)GTACCCCGTGTACCCCATTTCCATGGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605013733_xwim GGGACAAAGGGAAAAAAAAAACCAATTCAGAG(+ACTCAC)GTACCCATATGCAGAGGTCAGCCTACAACAC NGG 10 8 17 8 24 0 100 5 10
    240605013731_2vp2 TGAATGTGTTGTAGGCTGACCTCTGCATATGG(+ACTCAC)GTACCTCTGAATTGGTTTTTTTTTTCCCTTT NGG 10 8 17 8 24 0 100 5 10