Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013701_ypbw GGGCTTCCCTTGGCATCCCCCCATGGTACTAG(+ACTCAC)GTACCTTTGACCTATGAACCTGGGAGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605013700_vbog TCTTCCTCACCTCCCAGGTTCATAGGTCAAAG(+ACTCAC)GTACCTAGTACCATGGGGGGATGCCAAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605013658_q0w8 CCCCCAGGATTGGAATGGCTGAAAAGGGAGGG(+ACTCAC)GATCCATCTCTTTCCCATAGTTTTGGGACCT NGG 10 8 17 8 24 0 100 5 10
    240605013657_keut TTATTAGGTCCCAAAACTATGGGAAAGAGATG(+ACTCAC)GATCCCCTCCCTTTTCAGCCATTCCAATCCT NGG 10 8 17 8 24 0 100 5 10
    240605013655_arkv TAGAGTATTCACTAAAGGTGGATGTGCCTCAG(+ACTCAC)GTACCACAGTAACGCAGTCTTCCAAGTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605013654_f9u9 GAGCTGTTAACTTGGAAGACTGCGTTACTGTG(+ACTCAC)GTACCTGAGGCACATCCACCTTTAGTGAATA NGG 10 8 17 8 24 0 100 5 10
    240605013652_x7sf AGAGCACATTCTTAGCATGCACAGAACCTTAG(+ACTCAC)GATCCATCGCCAGCAACACACACAATGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605013651_b4ic TTTGTTTTTCATTGTGTGTGTTGCTGGCGATG(+ACTCAC)GATCCTAAGGTTCTGTGCATGCTAAGAATGT NGG 10 8 17 8 24 0 100 5 10
    240605013649_v011 CTCCAGAGTGCCCTGCAGCAATGCCCAGGAGG(+ACTCAC)GATCCAGACGGCTCCATTTGAGCTAAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605013648_ir4f CCCTGGCTGCTTAGCTCAAATGGAGCCGTCTG(+ACTCAC)GATCCCTCCTGGGCATTGCTGCAGGGCACTC NGG 10 8 17 8 24 0 100 5 10