Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013729_skp2 TGTCAAAGCATCCCCGAACTTCCTGCCCTAGG(+ACTCAC)GATCCAGGAGGTAGCCATCCAGGAGGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605013727_yopr ATGACAACCTCCTCCTGGATGGCTACCTCCTG(+ACTCAC)GATCCCTAGGGCAGGAAGTTCGGGGATGCTT NGG 10 8 17 8 24 0 100 5 10
    240605013726_1u31 AGTTTAATTGGCTAAGCCCTTCAGCCCTTTAG(+ACTCAC)GTACCTCATTAAAATGGAGATCTGTCTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605013725_qr23 AGAGTCTCAAGACAGATCTCCATTTTAATGAG(+ACTCAC)GTACCTAAAGGGCTGAAGGGCTTAGCCAATT NGG 10 8 17 8 24 0 100 5 10
    240605013723_igj0 GGGATGGATGAAGTCGGTGCTGGCCATGCCTG(+ACTCAC)GTACCATGATACTGGTTCTGGGCCAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605013721_zmm1 AGCCACCTCTCTGGCCCAGAACCAGTATCATG(+ACTCAC)GTACCAGGCATGGCCAGCACCGACTTCATCC NGG 10 8 17 8 24 0 100 5 10
    240605013720_k9qg GGCTCTCACATCTATGAGATGGGGCTAAAATG(+ACTCAC)GTACCTGTCCCTGTAGACTAACGGTATGAGG NGG 10 8 17 8 24 0 100 5 10
    240605013719_smbp TTAATCCTCATACCGTTAGTCTACAGGGACAG(+ACTCAC)GTACCATTTTAGCCCCATCTCATAGATGTGA NGG 10 8 17 8 24 0 100 5 10
    240605013718_15tf CCTGAAAGACTACTTGCTCTCTGCTATAGAAG(+ACTCAC)GATCCAGCACCCCTGTTCCCCACTTATAACT NGG 10 8 17 8 24 0 100 5 10
    240605013716_4xnq CTGTAAGTTATAAGTGGGGAACAGGGGTGCTG(+ACTCAC)GATCCTTCTATAGCAGAGAGCAAGTAGTCTT NGG 10 8 17 8 24 0 100 5 10