Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013614_wd0d TGGAGATCAGTCAACAAAATTGTAAATCCTTG(+ACTCAC)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605013613_y6vj CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+ACTCAC)GATCCAAGGATTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605013612_vay3 CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+ACTCAC)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605013610_f7cd TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+ACTCAC)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605013608_ejx6 TGGAGATCAGTCAACAAAATTGTAAATCCTTG(+ACTCAC)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605013607_15q8 CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+ACTCAC)GATCCAAGGATTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605013605_8wmg CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+ACTCAC)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605013604_5stl TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+ACTCAC)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605013602_dhqc CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+ACTCAC)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605013600_z77x TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+ACTCAC)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10