Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605014037_j2ty TGAACATTGCTACGATAAATATAGCAAAGCTG(+ACTCAC)GTACCATTTTATAAGCTGAGTACATTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605014036_obvw TTGAAAAATAAAATTAATAATTTAATTGGATG(+ACTCAC)GTACCAGTAACTAAACCGACTCCTGTTCATT NGG 10 8 17 8 24 0 100 5 10
    240605014035_ksg3 CCTTCAATGAACAGGAGTCGGTTTAGTTACTG(+ACTCAC)GTACCATCCAATTAAATTATTAATTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605014033_z9j7 AGTGTGAAATAAACAGGATAAAAAAGAAAATG(+ACTCAC)GTACCATGAGCAGGCAGAATGCTGCAATAAT NGG 10 8 17 8 24 0 100 5 10
    240605014032_z318 TATCAATTATTGCAGCATTCTGCCTGCTCATG(+ACTCAC)GTACCATTTTCTTTTTTATCCTGTTTATTTC NGG 10 8 17 8 24 0 100 5 10
    240605014031_x4oi GCAATCACATCGTGGCCAACCATCTGTAATGG(+ACTCAC)GATCCAATGCCCTCTTCAGGTATGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605014029_y2vq GCTGTCTTCAGACATACCTGAAGAGGGCATTG(+ACTCAC)GATCCCATTACAGATGGTTGGCCACGATGTG NGG 10 8 17 8 24 0 100 5 10
    240605014027_9q7g TAGCATTCAAGCCATTCTTAGGAAGGACCAGG(+ACTCAC)GATCCTCTCTCATCTCTTGGGGTTGCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240605014026_gxi7 CCTAGGAAAGGCAACCCCAAGAGATGAGAGAG(+ACTCAC)GATCCCTGGTCCTTCCTAAGAATGGCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605014025_dhlt GCAGTAGATAATCAGGAAGATACAATGACTTG(+ACTCAC)GATCCTAGAACCACCTTCCTTTTCCTTTATG NGG 10 8 17 8 24 0 100 5 10