Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013528_lo69 GTAGGCTTTTATATAAGGCAAAAAGCAAGATG(+ACTCAC)GATCCACTACTTTCCACAGAAAGAAACAGCT NGG 10 8 17 8 24 0 100 5 10
    240605013526_9m2j TATAGAGCTGTTTCTTTCTGTGGAAAGTAGTG(+ACTCAC)GATCCATCTTGCTTTTTGCCTTATATAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605013525_kkgp TGGTTGTTTGAATGCAGGTGGGCTGAGGTGGG(+ACTCAC)GATCCCCGAGAAAGGGTGCGTGCCACGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605013523_c7xk GCTTCTCTCCGTGGCACGCACCCTTTCTCGGG(+ACTCAC)GATCCCCACCTCAGCCCACCTGCATTCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605013521_2fhe GAGCTCCATTAAAGTCTCATGACATGCAAGTG(+ACTCAC)GATCCTCAGAGCAGATCCCCGTGAGGCATAG NGG 10 8 17 8 24 0 100 5 10
    240605013520_v2xq TCCCTCTATGCCTCACGGGGATCTGCTCTGAG(+ACTCAC)GATCCACTTGCATGTCATGAGACTTTAATGG NGG 10 8 17 8 24 0 100 5 10
    240605013519_rcvu CCATAAACCTGGAATATGTGTTTATCAGTAGG(+ACTCAC)GTACCCTACTTGAGCAGGAACCCTGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605013517_idmf ACCAATTTATTCAGGGTTCCTGCTCAAGTAGG(+ACTCAC)GTACCCTACTGATAAACACATATTCCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605013515_ky18 AGAACTTGTAGCACCTTTAACAGATTCCAAGG(+ACTCAC)GATCCAGTGTCTTCTCTTGGTTTCTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605013514_j3nu GAAATGCCTGAAGAAACCAAGAGAAGACACTG(+ACTCAC)GATCCCTTGGAATCTGTTAAAGGTGCTACAA NGG 10 8 17 8 24 0 100 5 10