Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013923_kx50 CAGGAACCCCTGGAGGCTAGAAGAGGGCATTG(+ACTCAC)GATCCTCTGGAGCTGGAACTGTAGGTGACTT NGG 10 8 17 8 24 0 100 5 10
    240605013922_wbu3 CCAGTGGGTCAGAAAACCCATGCTGTATCAAG(+ACTCAC)GTACCAAGATTATCGGATACTGTGAAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605013920_0jgi TATAAGTCACTTCACAGTATCCGATAATCTTG(+ACTCAC)GTACCTTGATACAGCATGGGTTTTCTGACCC NGG 10 8 17 8 24 0 100 5 10
    240605013919_rsih GCTGGGCAGGTCAGTGGCAGAGGTGGAACACG(+ACTCAC)GATCCCTTCCTGACCGCGTGGTAGGTAACTT NGG 10 8 17 8 24 0 100 5 10
    240605013917_5fu1 GTCGAAAGTTACCTACCACGCGGTCAGGAAGG(+ACTCAC)GATCCGTGTTCCACCTCTGCCACTGACCTGC NGG 10 8 17 8 24 0 100 5 10
    240605013916_6w2u AGGAGTTAGTTAAATGCTGAAGAGGCTCTCAG(+ACTCAC)GTACCCACATCCTGAACCGTGGGGGCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605013915_aedt CCTGGGCTCAGCCCCCACGGTTCAGGATGTGG(+ACTCAC)GTACCTGAGAGCCTCTTCAGCATTTAACTAA NGG 10 8 17 8 24 0 100 5 10
    240605013913_fknb CAGGACAGGTTAAAAGGAAACAGCCAAGTGAG(+ACTCAC)GTACCTTAGCTGGCAGCAGCGCGAGGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240605013912_np0b CCTGAACCGTGGGGGCTGAGCCCAGGTGGATG(+ACTCAC)GATCCAGTGCAGAGTTACCAACCTCCTCGAT NGG 10 8 17 8 24 0 100 5 10
    240605013910_upx5 TGCCCATCGAGGAGGTTGGTAACTCTGCACTG(+ACTCAC)GATCCATCCACCTGGGCTCAGCCCCCACGGT NGG 10 8 17 8 24 0 100 5 10