Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013416_t016 CGAGCCCTCTAAGTTGGCGTTTGCTACTAAAG(+ACTCAC)GATCCCCAGAATCCCCAATACTGTAACAACG NGG 10 8 17 8 24 0 100 5 10
    240605013414_9gbd ACCACCGTTGTTACAGTATTGGGGATTCTGGG(+ACTCAC)GATCCTTTAGTAGCAAACGCCAACTTAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605013412_jajc AATTCCACTAGTTGCTGTCGACCATATTCTTG(+ACTCAC)GTACCCCACCCCCACCCCTTACTATGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605013410_pss5 CTTAGTGCAGCATAGTAAGGGGTGGGGGTGGG(+ACTCAC)GTACCAAGAATATGGTCGACAGCAACTAGTG NGG 10 8 17 8 24 0 100 5 10
    240605013409_1dm0 GTCCGAAAGCAGCATTAACAATCAAGGTTCTG(+ACTCAC)GTACCTGTGCGGGCTCATGGTTGCCTTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605013407_aho0 GAGTTGCCCAAGGCAACCATGAGCCCGCACAG(+ACTCAC)GTACCAGAACCTTGATTGTTAATGCTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605013405_9885 GAATGTTTTTCAGAGACCGCTCTGAAACCCTG(+ACTCAC)GTACCACTGTCCTCTACGGGCTAAGAATCTA NGG 10 8 17 8 24 0 100 5 10
    240605013404_3o38 AGCGTTAGATTCTTAGCCCGTAGAGGACAGTG(+ACTCAC)GTACCAGGGTTTCAGAGCGGTCTCTGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605013402_ku4i TTAAAGAAACATTTGTCCAAATAGGCAAAGTG(+ACTCAC)GATCCCGACTTCAAACACATGAACTAGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605013401_9o71 AATGTTCTACTAGTTCATGTGTTTGAAGTCGG(+ACTCAC)GATCCACTTTGCCTATTTGGACAAATGTTTC NGG 10 8 17 8 24 0 100 5 10