Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013330_jcua TTTTTAAAAACCTGTCACTACCAGAAACGGTG(+ACTCAC)GTACCTCCTGCCCTTTGACTCTCATACCGTG NGG 10 8 17 8 24 0 100 5 10
    240605013329_gr8q ACTTTCACGGTATGAGAGTCAAAGGGCAGGAG(+ACTCAC)GTACCACCGTTTCTGGTAGTGACAGGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605013328_a09y CTAATGCAGCCCAGTAGAGTGGAGCTCAGGAG(+ACTCAC)GTACCCACAGACGGGATTTCTCTTGGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605013326_kotg TCAGTCAAGACCAAGAGAAATCCCGTCTGTGG(+ACTCAC)GTACCTCCTGAGCTCCACTCTACTGGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605013323_akfs GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+ACTCAC)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605013322_ww60 GCAAACAGCAAGAGGCTTTATTGGGAACACGG(+ACTCAC)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240605013320_4ffw TAGAAGCAGGTTCTTGGAAGTTACAATTTAAG(+ACTCAC)GTACCCCCAAAAAGTTGGAAATAAAAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605013318_i3ly ATTTGTTTCCTTTTATTTCCAACTTTTTGGGG(+ACTCAC)GTACCTTAAATTGTAACTTCCAAGAACCTGC NGG 10 8 17 8 24 0 100 5 10
    240605013317_n7fj ACATACACAGCTACAGAGTGAGAGTTGGTTTG(+ACTCAC)GATCCTCTTCATTTCATTTGTTTGCCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605013316_bv1e ATAACAGAAAGGCAAACAAATGAAATGAAGAG(+ACTCAC)GATCCAAACCAACTCTCACTCTGTAGCTGTG NGG 10 8 17 8 24 0 100 5 10