Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013559_jdjj CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+ACTCAC)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605013557_i1h3 TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+ACTCAC)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605013556_1841 CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+ACTCAC)GATCCAAGGTTTTTCAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605013554_cxb6 TGGAGATCAGTCAACAAAATTGAAAAACCTTG(+ACTCAC)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605013553_zst3 CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+ACTCAC)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605013551_h45k TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+ACTCAC)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605013549_aqd7 GGAATGACCATCCAGAGACTGCTTCACCTGGG(+ACTCAC)GATCCATCTCATAAACAACCAGAAAAACCAG NGG 10 8 17 8 24 0 100 5 10
    240605013547_bc1g AGTGTCTGGTTTTTCTGGTTGTTTATGAGATG(+ACTCAC)GATCCCCAGGTGAAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605013546_a5j7 CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+ACTCAC)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605013544_4mou TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+ACTCAC)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10