Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013215_8yeu CCTCAGAAGCTATGTATTAAGCTGTGCTCGCG(+ACTCAC)GTACCTAATACAAAATACACTATATTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240605013214_m7xt GGCAAATCAAAATATAGTGTATTTTGTATTAG(+ACTCAC)GTACCGCGAGCACAGCTTAATACATAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605013213_x2mz CTTTTACTTGCCTGAGTCAACATTTTTAAAGG(+ACTCAC)GATCCATTTATTCTTCCTTTGTAGTTTTCGC NGG 10 8 17 8 24 0 100 5 10
    240605013211_i38p GTAAAGCGAAAACTACAAAGGAAGAATAAATG(+ACTCAC)GATCCCTTTAAAAATGTTGACTCAGGCAAGT NGG 10 8 17 8 24 0 100 5 10
    240605013209_zfip GCTACTGTACTTGCCTCTGCCTGAGCACCAGG(+ACTCAC)GATCCAAATCATTGTATAACACACCTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605013207_x54b TCTCTCCAGGAGGTGTGTTATACAATGATTTG(+ACTCAC)GATCCCTGGTGCTCAGGCAGAGGCAAGTACA NGG 10 8 17 8 24 0 100 5 10
    240605013206_r6jq CTCCTCGTGTTCTGTTAGAACTCAAGGACAAG(+ACTCAC)GTACCACAGAGAACACCTGCAATGTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605013205_0obw GTGACAAAGAAACATTGCAGGTGTTCTCTGTG(+ACTCAC)GTACCTTGTCCTTGAGTTCTAACAGAACACG NGG 10 8 17 8 24 0 100 5 10
    240605013204_xmsl CCACCTGAGAGCAGGGACAACAGAACAGACAG(+ACTCAC)GTACCCATCAGTTCTGCTCCAGCAAGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605013202_tpj1 CAGTAGCTTGCTTGCTGGAGCAGAACTGATGG(+ACTCAC)GTACCTGTCTGTTCTGTTGTCCCTGCTCTCA NGG 10 8 17 8 24 0 100 5 10