Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013117_g843 CCCCAAGATCTTTGAGATGTCAGAGCTGTGGG(+ACTCAC)GTACCTGCTCAGGAAAACCAGCCAAAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605013116_5wsq ATTCTTTCTCTTTGGCTGGTTTTCCTGAGCAG(+ACTCAC)GTACCCCACAGCTCTGACATCTCAAAGATCT NGG 10 8 17 8 24 0 100 5 10
    240605013115_odat TCTCTTTGGAGGACTGCAGAAGATCATGTGTG(+ACTCAC)GATCCAAGACATTGGAGCAAGAAGCTGTAAC NGG 10 8 17 8 24 0 100 5 10
    240605013114_9zb7 TCAGTGTTACAGCTTCTTGCTCCAATGTCTTG(+ACTCAC)GATCCACACATGATCTTCTGCAGTCCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605013112_eejr CCCCAAGATCTTTGAGATGCCAGAGCTGTGGG(+ACTCAC)GTACCTGCTCATGAAAACCAGCCAAAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605013111_apqh ATTCTTTCTCTTTGGCTGGTTTTCATGAGCAG(+ACTCAC)GTACCCCACAGCTCTGGCATCTCAAAGATCT NGG 10 8 17 8 24 0 100 5 10
    240605013110_nrgj TCTCTTTGGAGGATAGCAGAAGATCATGTGTG(+ACTCAC)GATCCAAGACATTGGAACAAGAAACTGTAAC NGG 10 8 17 8 24 0 100 5 10
    240605013109_cioo TCAGTGTTACAGTTTCTTGTTCCAATGTCTTG(+ACTCAC)GATCCACACATGATCTTCTGCTATCCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605013107_jjws CACAGAAGTCATCACATAGGGAAGATATGAAG(+ACTCAC)GATCCTTTAGAGAAAACTTTAACAAAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605013106_c4j6 CTTGTAAAACTTTGTTAAAGTTTTCTCTAAAG(+ACTCAC)GATCCTTCATATCTTCCCTATGTGATGACTT NGG 10 8 17 8 24 0 100 5 10