Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013105_r19a AGACAGATGCCTCCACAGTTACTGCCAAACTG(+ACTCAC)GTACCTAAGGACCAAAATAGCAGTCAGATGA NGG 10 8 17 8 24 0 100 5 10
    240605013103_px8f TGTGGTCATCTGACTGCTATTTTGGTCCTTAG(+ACTCAC)GTACCAGTTTGGCAGTAACTGTGGAGGCATC NGG 10 8 17 8 24 0 100 5 10
    240605013100_qgte GATGTCATTTTAGCAGATTTTGTGTTTTGGGG(+ACTCAC)GTACCACACCCTATGGCAAGCTTGGATACCT NGG 10 8 17 8 24 0 100 5 10
    240605013059_csj7 GCACCAGGTATCCAAGCTTGCCATAGGGTGTG(+ACTCAC)GTACCCCCAAAACACAAAATCTGCTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240605013058_u77w CTTAAACTAGAAATCCTGCTCCATTAGCACAG(+ACTCAC)GATCCACTAAACAGGTAAAAGCCTGCTGCGC NGG 10 8 17 8 24 0 100 5 10
    240605013057_rl2i GCAGAGCGCAGCAGGCTTTTACCTGTTTAGTG(+ACTCAC)GATCCTGTGCTAATGGAGCAGGATTTCTAGT NGG 10 8 17 8 24 0 100 5 10
    240605013055_603t ATGGCAGCTCCAAATAATCTGGTGAAATTCCG(+ACTCAC)GATCCCAGCATTGTATGGTGGCTGGACTTCA NGG 10 8 17 8 24 0 100 5 10
    240605013053_h7cg ATTTCTGAAGTCCAGCCACCATACAATGCTGG(+ACTCAC)GATCCGGAATTTCACCAGATTATTTGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605013051_u4qc TAAGTACCCCAGTGAGACTGTAATTACCTTAG(+ACTCAC)GATCCTCAAAATGTGTTCATAAACTCAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605013050_c4rh TAAGCAAGCTGAGTTTATGAACACATTTTGAG(+ACTCAC)GATCCTAAGGTAATTACAGTCTCACTGGGGT NGG 10 8 17 8 24 0 100 5 10