Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013008_6kfc TTAAAAGGCACTTATAATAAAGAGAGCACATG(+ACTCAC)GATCCATCCACTAGACTAAGGCGGGAATGGA NGG 10 8 17 8 24 0 100 5 10
    240605013007_bj8i AATTTTCCATTCCCGCCTTAGTCTAGTGGATG(+ACTCAC)GATCCATGTGCTCTCTTTATTATAAGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605013005_oh1z ATCTTTAAAAAATATTGATGGTATATTGCTTG(+ACTCAC)GTACCAAAAGAAAAGAAAAAATGAAATTCAC NGG 10 8 17 8 24 0 100 5 10
    240605013004_5yv1 CCTGTGTGAATTTCATTTTTTCTTTTCTTTTG(+ACTCAC)GTACCAAGCAATATACCATCAATATTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605013003_d7ck TTTGGTTATTCAGATCTTATCCAACACAATTG(+ACTCAC)GATCCATACTAAAAAGAAACCAAACCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605013002_tzzh TCTTCACAAAGGTTTGGTTTCTTTTTAGTATG(+ACTCAC)GATCCAATTGTGTTGGATAAGATCTGAATAA NGG 10 8 17 8 24 0 100 5 10
    240605013000_5b8g TGGGAGGAACCAACCCATCTCTGTTGAGCAAG(+ACTCAC)GATCCTGGATTGTATAAAAGGAGAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605012958_vs1x CTCAGTTCCCTTTCTCCTTTTATACAATCCAG(+ACTCAC)GATCCTTGCTCAACAGAGATGGGTTGGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605012957_m53s ATGCAGTTCTTACATAAATAACTATTGATAGG(+ACTCAC)GATCCTAAAAGTGATCTCACAGTTGTGTTTA NGG 10 8 17 8 24 0 100 5 10
    240605012955_21lx ATGAATAAACACAACTGTGAGATCACTTTTAG(+ACTCAC)GATCCCTATCAATAGTTATTTATGTAAGAAC NGG 10 8 17 8 24 0 100 5 10