Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012905_039t GTTAAAGGACTCCTGAGAAGTTACAGACTCAG(+ACTCAC)GATCCTTATAATTATTTGGGTTTTAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240605012904_usvm ATCTGTAAGTCTAAAACCCAAATAATTATAAG(+ACTCAC)GATCCTGAGTCTGTAACTTCTCAGGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605012902_617d ATCCCTTCTACTAAGTGCATACATGTAGTTTG(+ACTCAC)GATCCATATTTCAATTCACTGTCAAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605012901_iozc GTCATAGAAACTTGACAGTGAATTGAAATATG(+ACTCAC)GATCCAAACTACATGTATGCACTTAGTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605012900_8xst ACAAGACCAAAGGACAAAGGAAAAAAATCTTG(+ACTCAC)GATCCACAAAATTCTGAAAATTATTGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605012858_7kwb AATACCTCCTCAATAATTTTCAGAATTTTGTG(+ACTCAC)GATCCAAGATTTTTTTCCTTTGTCCTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605012857_7gjd TGTGTGTGAAACCAGAGATGAAAGCATGATTG(+ACTCAC)GATCCTTTATACTTGGTAAACAAACCTCATG NGG 10 8 17 8 24 0 100 5 10
    240605012856_gg9m ATAGACATGAGGTTTGTTTACCAAGTATAAAG(+ACTCAC)GATCCAATCATGCTTTCATCTCTGGTTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605012855_lx4s ATCCCTTCTACTAAGTGCATACATGTAGTTTG(+ACTCAC)GATCCATATTTCAATTCACTGTCAAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605012853_xk17 GTCATAGAAACTTGACAGTGAATTGAAATATG(+ACTCAC)GATCCAAACTACATGTATGCACTTAGTAGAA NGG 10 8 17 8 24 0 100 5 10