Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012806_a4m7 GATCCCTCTGCACCACGTGTCTTAGAATTAAG(+ACTCAC)GATCCAAAAGAAGAAAAAAAGTTACCATGGT NGG 10 8 17 8 24 0 100 5 10
    240605012805_cvm3 TCAACACCATGGTAACTTTTTTTCTTCTTTTG(+ACTCAC)GATCCTTAATTCTAAGACACGTGGTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605012803_wj3l TCAACCTTCTGAGGCATGTGCTACCATGCCTG(+ACTCAC)GATCCCAAGTTTTTCCTTAAGTGGTCTTGAT NGG 10 8 17 8 24 0 100 5 10
    240605012801_2kqh AGTCAATCAAGACCACTTAAGGAAAAACTTGG(+ACTCAC)GATCCAGGCATGGTAGCACATGCCTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605012759_pnh5 GGAACTTTTCAGAAGATGCTTAGTGAAAGGGG(+ACTCAC)GATCCGTTCAAATAGATGTCTGGTCCCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605012757_7bzx ACCCTTGCAGGGACCAGACATCTATTTGAACG(+ACTCAC)GATCCCCCTTTCACTAAGCATCTTCTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605012755_pzex ACATTCTACCCTTTGGGTTCTAAGATTCCAGG(+ACTCAC)GTACCAATAGCCATGCATGACAGCTGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605012754_n6nq TGGTATGGCTCAGCTGTCATGCATGGCTATTG(+ACTCAC)GTACCCTGGAATCTTAGAACCCAAAGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605012753_ezwp CTTGTGGGAGAGTTTTCGCTTTCCAGCATGTG(+ACTCAC)GTACCAGGAATAGCATCAGGTTGTCAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605012751_slh2 CTGCTGAGCCTGACAACCTGATGCTATTCCTG(+ACTCAC)GTACCACATGCTGGAAAGCGAAAACTCTCCC NGG 10 8 17 8 24 0 100 5 10