Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012852_gwwj TGTTTAATGTGAACTGATATATTTAAAACAAG(+ACTCAC)GATCCAGGGTAGACAGGGAGAATGTTTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605012850_8f2s GTTCTACTAAAACATTCTCCCTGTCTACCCTG(+ACTCAC)GATCCTTGTTTTAAATATATCAGTTCACATT NGG 10 8 17 8 24 0 100 5 10
    240605012848_4spg AAAAGGATATACCTTCTTCTCAGCACTTCATG(+ACTCAC)GTACCTCCTCCTAAATTGACCATATACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605012847_y5a3 TGTGACTGGGTATATGGTCAATTTAGGAGGAG(+ACTCAC)GTACCATGAAGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240605012845_px3h ATGAAATGGACAACTTCCTAGACAGATACCAG(+ACTCAC)GTACCAAAGTTAAGTCAGAATCAGATTAATG NGG 10 8 17 8 24 0 100 5 10
    240605012844_uu9p TAGATCATTAATCTGATTCTGACTTAACTTTG(+ACTCAC)GTACCTGGTATCTGTCTAGGAAGTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605012842_a543 GGATAAAGGAACTCCGCCGGACTAGAGGCTGG(+ACTCAC)GATCCATTCCGGTTGGGGCCAGCCCCGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605012841_vez1 GGAAGATGGCGGGGCTGGCCCCAACCGGAATG(+ACTCAC)GATCCCAGCCTCTAGTCCGGCGGAGTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605012839_1h00 TTCTTTCTTTGTGTTGGTTGGACTTTAAGAGG(+ACTCAC)GTACCAAGCTGGAGTGGATGCATAAGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605012838_73b0 TTTCTACTTCCTTATGCATCCACTCCAGCTTG(+ACTCAC)GTACCCTCTTAAAGTCCAACCAACACAAAGA NGG 10 8 17 8 24 0 100 5 10