Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012954_bquu TTTGCTGAGTCAGGATTTATATGGATACAAAG(+ACTCAC)GATCCCTTCTGAAAACTGTCCATTGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605012952_ohgy GTACGCCTGCACAATGGACAGTTTTCAGAAGG(+ACTCAC)GATCCTTTGTATCCATATAAATCCTGACTCA NGG 10 8 17 8 24 0 100 5 10
    240605012951_vjoe GAATAGTGGCTGTTCATTTATACCCTCAGCTG(+ACTCAC)GTACCAACTGAGAAGCTTGCTGTCTGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605012950_mh89 AGGTAGCCAGCAGACAGCAAGCTTCTCAGTTG(+ACTCAC)GTACCAGCTGAGGGTATAAATGAACAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605012949_uin6 GAAACTATTCCTTTGGAGGAGTCCTGATTGTG(+ACTCAC)GATCCCAAATGTTGTAGACTTTGAACTTTGC NGG 10 8 17 8 24 0 100 5 10
    240605012947_7xrm TAAAAGCAAAGTTCAAAGTCTACAACATTTGG(+ACTCAC)GATCCACAATCAGGACTCCTCCAAAGGAATA NGG 10 8 17 8 24 0 100 5 10
    240605012945_m0f6 TCACAACTAAGGTTACCCCCATTGATTCTTGG(+ACTCAC)GTACCTCCCCTATCCCATACCTCCCTCATGT NGG 10 8 17 8 24 0 100 5 10
    240605012943_ebf5 TGAAGACATGAGGGAGGTATGGGATAGGGGAG(+ACTCAC)GTACCCAAGAATCAATGGGGGTAACCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605012941_5xtt GTCAGGCCTTTCACACCAATACTCCCCTCCTG(+ACTCAC)GTACCAACTTCTTAGGTTGGGTTTTATCACC NGG 10 8 17 8 24 0 100 5 10
    240605012940_oy58 CTTCAGGTGATAAAACCCAACCTAAGAAGTTG(+ACTCAC)GTACCAGGAGGGGAGTATTGGTGTGAAAGGC NGG 10 8 17 8 24 0 100 5 10