Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012700_olu0 ACGTGGTTGGGGGCTTCTGGATCCTGGTGTGG(+ACTCAC)GTACCAATCTCCCTTCATAGCTTTTCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605012659_k7z0 TAGGATCTGAGAAAAGCTATGAAGGGAGATTG(+ACTCAC)GTACCCACACCAGGATCCAGAAGCCCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240605012657_9af5 TCCTGGCCTCAGCACGTGGTTGGGGGCTTCTG(+ACTCAC)GATCCTGGTGTGGGTACCAATCTCCCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605012655_s7s9 AAGCTATGAAGGGAGATTGGTACCCACACCAG(+ACTCAC)GATCCAGAAGCCCCCAACCACGTGCTGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605012654_nlw2 TGGATGAAGCATCTTTAACCTCATAATAAATG(+ACTCAC)GATCCAGATTTTACCTCAAAGAATTATATTC NGG 10 8 17 8 24 0 100 5 10
    240605012653_f9lp AATAAGAATATAATTCTTTGAGGTAAAATCTG(+ACTCAC)GATCCATTTATTATGAGGTTAAAGATGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605012651_dvn3 ATCATAGAACAACTTGCACTTTCTGCTATATG(+ACTCAC)GATCCAAGATATTGAACTGAGTGATCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605012650_cr84 TTTCAAACCTGATCACTCAGTTCAATATCTTG(+ACTCAC)GATCCATATAGCAGAAAGTGCAAGTTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605012648_hrm8 TTTTCATCCTCCTGGGGATGTGGAAGCTGCAG(+ACTCAC)GATCCCTGAGATGAAGTGCAGTGTGGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605012647_zjbc GACACAGGCTCCACACTGCACTTCATCTCAGG(+ACTCAC)GATCCTGCAGCTTCCACATCCCCAGGAGGAT NGG 10 8 17 8 24 0 100 5 10