Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012628_rj9b CTGGTCCAGGGAAGGAACAGGTGTTAGGATGG(+ACTCAC)GTACCCAAGCTACAGATCTGATGAGGGTAAC NGG 10 8 17 8 24 0 100 5 10
    240605012627_0evx ATGGGGTTACCCTCATCAGATCTGTAGCTTGG(+ACTCAC)GTACCCATCCTAACACCTGTTCCTTCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605012625_bjb1 CACGGCAAGAGCAAGGCTGCAAACTGTCTCTG(+ACTCAC)GTACCACGGAATGTGCTTTCCAACACACATG NGG 10 8 17 8 24 0 100 5 10
    240605012623_ea33 CAGCCCATGTGTGTTGGAAAGCACATTCCGTG(+ACTCAC)GTACCAGAGACAGTTTGCAGCCTTGCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605012622_lnu8 GTTCCTCCACAGATGTGCTAAAGTCCAAAGAG(+ACTCAC)GATCCATTTGGAAGGAGTGGCTTGGCCGGGG NGG 10 8 17 8 24 0 100 5 10
    240605012621_mdqx CACGGCCCCGGCCAAGCCACTCCTTCCAAATG(+ACTCAC)GATCCTCTTTGGACTTTAGCACATCTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605012620_0fu2 GAGTGGGTAGTTGTGGACTTGTTAACAGTAAG(+ACTCAC)GTACCTTTGAAGTATGCAGATGGAGGTGACC NGG 10 8 17 8 24 0 100 5 10
    240605012619_1okg CACTGGGTCACCTCCATCTGCATACTTCAAAG(+ACTCAC)GTACCTTACTGTTAACAAGTCCACAACTACC NGG 10 8 17 8 24 0 100 5 10
    240605012617_ygfq GACCGAGCTATGCATGGAGGAGGAGGCTGTTG(+ACTCAC)GTACCATACTGAATTTCCACTGGACCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240605012616_evyz TGACTTTAGAGGTCCAGTGGAAATTCAGTATG(+ACTCAC)GTACCAACAGCCTCCTCCTCCATGCATAGCT NGG 10 8 17 8 24 0 100 5 10