Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605013055_603t ATGGCAGCTCCAAATAATCTGGTGAAATTCCG(+ACTCAC)GATCCCAGCATTGTATGGTGGCTGGACTTCA NGG 10 8 17 8 24 0 100 5 10
    240605013053_h7cg ATTTCTGAAGTCCAGCCACCATACAATGCTGG(+ACTCAC)GATCCGGAATTTCACCAGATTATTTGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605013051_u4qc TAAGTACCCCAGTGAGACTGTAATTACCTTAG(+ACTCAC)GATCCTCAAAATGTGTTCATAAACTCAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605013050_c4rh TAAGCAAGCTGAGTTTATGAACACATTTTGAG(+ACTCAC)GATCCTAAGGTAATTACAGTCTCACTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240605013048_wrwn CCTTTGTATCCTCATGGTTCTTTGACCTTCGG(+ACTCAC)GTACCTGAATAGAAAAGGCAATGCTTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605013047_wp4j ACTTCACAAAAAGCATTGCCTTTTCTATTCAG(+ACTCAC)GTACCCGAAGGTCAAAGAACCATGAGGATAC NGG 10 8 17 8 24 0 100 5 10
    240605013046_lw8x AGACACCCCCATGTGACTAGGGAGTGAGTCTG(+ACTCAC)GTACCTCACTCAGGATGATAGCATTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605013045_t2dj TATCTGAAGGAAATGCTATCATCCTGAGTGAG(+ACTCAC)GTACCAGACTCACTCCCTAGTCACATGGGGG NGG 10 8 17 8 24 0 100 5 10
    240605013044_0fvr CCCCTATTTTATCTTTATAGTGATCTCAGTAG(+ACTCAC)GTACCCACTATGGTTACTGTCAAACGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605013042_3tga TCCTTGGCAGCGTTTGACAGTAACCATAGTGG(+ACTCAC)GTACCTACTGAGATCACTATAAAGATAAAAT NGG 10 8 17 8 24 0 100 5 10