Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012523_nghd TCCAAGGGCTTCAGTCCAACTGTCCAAGATGG(+ACTCAC)GTACCAACCTTACTGCCTGGGGGTTATACAG NGG 10 8 17 8 24 0 100 5 10
    240605012521_2zao ATCTGCTGTATAACCCCCAGGCAGTAAGGTTG(+ACTCAC)GTACCCATCTTGGACAGTTGGACTGAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240605012520_fsnb TTCTACTCTTAGAAATACTGATAGCTTCTGAG(+ACTCAC)GTACCCATTTTCTAAAGACACCTGAAACAAA NGG 10 8 17 8 24 0 100 5 10
    240605012518_w3lt ATGGCTTTGTTTCAGGTGTCTTTAGAAAATGG(+ACTCAC)GTACCTCAGAAGCTATCAGTATTTCTAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605012517_ukc9 ATGGACTCCAGGAGTTACACCCTACCAAGAAG(+ACTCAC)GTACCCAGGGGAGCAGTGCAGACACCACACC NGG 10 8 17 8 24 0 100 5 10
    240605012514_04xd CTGCTGGTGTGGTGTCTGCACTGCTCCCCTGG(+ACTCAC)GTACCTTCTTGGTAGGGTGTAACTCCTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605012513_2o80 AAACATTCACAGTTTCACTTCCCTTGTGTGTG(+ACTCAC)GTACCTAGACAGGGCATCTTCTTCAGATAGT NGG 10 8 17 8 24 0 100 5 10
    240605012511_8d26 CCAGTACTATCTGAAGAAGATGCCCTGTCTAG(+ACTCAC)GTACCACACACAAGGGAAGTGAAACTGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605012510_y2aa AAGCCTAATAAATGTGCAAAGGTGGGGGGAAG(+ACTCAC)GATCCCACTTCCCATTTTCTATTTCACCTGT NGG 10 8 17 8 24 0 100 5 10
    240605012508_oa4u CACCAACAGGTGAAATAGAAAATGGGAAGTGG(+ACTCAC)GATCCTTCCCCCCACCTTTGCACATTTATTA NGG 10 8 17 8 24 0 100 5 10