Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012450_2q84 CAAAGGCAGCTGCAGAGCTCAGCCTTGGGTAG(+ACTCAC)GATCCTGTCTGAGGACACTGACTGAGGACAT NGG 10 8 17 8 24 0 100 5 10
    240605012449_pmjj CAGGGATGTCCTCAGTCAGTGTCCTCAGACAG(+ACTCAC)GATCCTACCCAAGGCTGAGCTCTGCAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605012447_c51r CTGCCATGGTCCTAGAAAATTTGCATCACCAG(+ACTCAC)GATCCTGGAGGGACAAGGGTGGAAAGTATTT NGG 10 8 17 8 24 0 100 5 10
    240605012445_g2ek ATTTGAAATACTTTCCACCCTTGTCCCTCCAG(+ACTCAC)GATCCTGGTGATGCAAATTTTCTAGGACCAT NGG 10 8 17 8 24 0 100 5 10
    240605012444_o3ll TCAGTGTACTGGAGGAGAAAATCAAACTGTTG(+ACTCAC)GATCCCAAGTGGCTACATCACCATGAAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605012442_lh15 CTGATTCTCTTCATGGTGATGTAGCCACTTGG(+ACTCAC)GATCCAACAGTTTGATTTTCTCCTCCAGTAC NGG 10 8 17 8 24 0 100 5 10
    240605012440_p3nx TTGAGTCCACTCAGTGCCTCCCTGGAGACCTG(+ACTCAC)GATCCATCCTGCCCTGGCCATTACCTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605012439_u645 CATGTCAACAAGGTAATGGCCAGGGCAGGATG(+ACTCAC)GATCCAGGTCTCCAGGGAGGCACTGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605012437_hbew TAATGCCTAAGCGCTCCTTGGCTCCCAGAGTG(+ACTCAC)GATCCTGCCTAGTTTGTCACTGTCACTTTAG NGG 10 8 17 8 24 0 100 5 10
    240605012435_lo2r TCTTTCTAAAGTGACAGTGACAAACTAGGCAG(+ACTCAC)GATCCACTCTGGGAGCCAAGGAGCGCTTAGG NGG 10 8 17 8 24 0 100 5 10