Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012507_df16 AACCAGACATGATACCCCAGGTTTTATTTCTG(+ACTCAC)GATCCCACATGGTGGAAGGGGAGAAGTGACC NGG 10 8 17 8 24 0 100 5 10
    240605012505_vvlg TGTTGGGTCACTTCTCCCCTTCCACCATGTGG(+ACTCAC)GATCCAGAAATAAAACCTGGGGTATCATGTC NGG 10 8 17 8 24 0 100 5 10
    240605012503_4pbr CTTTTGGCAATCCTATCGGAAAGAATTTTAGG(+ACTCAC)GATCCAGGAGAGCTACTAATACGAGATACCA NGG 10 8 17 8 24 0 100 5 10
    240605012501_2a6a GCAAATGGTATCTCGTATTAGTAGCTCTCCTG(+ACTCAC)GATCCCTAAAATTCTTTCCGATAGGATTGCC NGG 10 8 17 8 24 0 100 5 10
    240605012500_ggwa TAGCAACAAGAATCCAAGTGTACACTGTGGTG(+ACTCAC)GATCCAGCTTCAAAGCAAACTGTTGCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605012458_gdal GTGTTTGGAGGCAACAGTTTGCTTTGAAGCTG(+ACTCAC)GATCCACCACAGTGTACACTTGGATTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605012457_na3e GTGAAATCAAGATTAGTTAAACCATCTAAACG(+ACTCAC)GATCCATAACCCCTAAGGAAATACAAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605012456_ck2b TAATGACTCTTTGTATTTCCTTAGGGGTTATG(+ACTCAC)GATCCGTTTAGATGGTTTAACTAATCTTGAT NGG 10 8 17 8 24 0 100 5 10
    240605012453_gud3 GGAAAGACCATCCAGAGACTGTCCTACCAGGG(+ACTCAC)GATCCATCCCATATACAACCACCAAACACAG NGG 10 8 17 8 24 0 100 5 10
    240605012452_l4eq AGTGTCTGTGTTTGGTGGTTGTATATGGGATG(+ACTCAC)GATCCCCTGGTAGGACAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10