Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012348_oxph AAACATCTCCAAACCCATAAAGAGATCGCCTG(+ACTCAC)GTACCGAAACCAGATGAGAAAAGGGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605012347_9fyj GCTGCCTCCTCCCCTTTTCTCATCTGGTTTCG(+ACTCAC)GTACCAGGCGATCTCTTTATGGGTTTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605012344_j0sl CCAGCAAGGCTGATAATGTGCTTGATCCCCAG(+ACTCAC)GATCCACAGCGTGGAAGGAAAGAACCCATGT NGG 10 8 17 8 24 0 100 5 10
    240605012343_wf93 TGTAGACATGGGTTCTTTCCTTCCACGCTGTG(+ACTCAC)GATCCTGGGGATCAAGCACATTATCAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605012341_h4rn TTGGTTTGGATGGAAGATGAGCAGCCTGGTGG(+ACTCAC)GATCCTTTGGAGGGTAAGATGGTGATGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605012339_9pdw ACTCTAAAACATCACCATCTTACCCTCCAAAG(+ACTCAC)GATCCCACCAGGCTGCTCATCTTCCATCCAA NGG 10 8 17 8 24 0 100 5 10
    240605012337_hust TAAAGTGATGGAGGGCAACGGAGGGTGATGGG(+ACTCAC)GATCCTGAAGCCAGGAGTCAGAAATCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605012335_29s4 ATGACACCAAGATTTCTGACTCCTGGCTTCAG(+ACTCAC)GATCCCCATCACCCTCCGTTGCCCTCCATCA NGG 10 8 17 8 24 0 100 5 10
    240605012333_3sgh CAGCTGTAACCTTCAGTCTTCCACATAGCCTG(+ACTCAC)GTACCTACTGACTCGATATTGACCACTGACA NGG 10 8 17 8 24 0 100 5 10
    240605012332_z155 AACCATGTCAGTGGTCAATATCGAGTCAGTAG(+ACTCAC)GTACCAGGCTATGTGGAAGACTGAAGGTTAC NGG 10 8 17 8 24 0 100 5 10