Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012242_ccgi GCCATGCACAGTGGTACACATCTGCAATCCTG(+ACTCAC)GTACCTGACAAGCTGAGACAGAAGGATCACA NGG 10 8 17 8 24 0 100 5 10
    240605012241_vr0e GAACTTGTGATCCTTCTGTCTCAGCTTGTCAG(+ACTCAC)GTACCAGGATTGCAGATGTGTACCACTGTGC NGG 10 8 17 8 24 0 100 5 10
    240605012239_69wy GCCACCATACAGGATCACCTACAGAAAACAGG(+ACTCAC)GATCCTCAAGGGAGGACAAATCCCCCCACAG NGG 10 8 17 8 24 0 100 5 10
    240605012238_ysxu ACGTTCTGTGGGGGGATTTGTCCTCCCTTGAG(+ACTCAC)GATCCCTGTTTTCTGTAGGTGATCCTGTATG NGG 10 8 17 8 24 0 100 5 10
    240605012237_qa6w CGGGCCCTGTCCTGCTCTCGCATTGCTTGATG(+ACTCAC)GATCCTACACCAGTCTAAAAACTTACATAAG NGG 10 8 17 8 24 0 100 5 10
    240605012236_jbrs GTCTGCTTATGTAAGTTTTTAGACTGGTGTAG(+ACTCAC)GATCCATCAAGCAATGCGAGAGCAGGACAGG NGG 10 8 17 8 24 0 100 5 10
    240605012234_3vi3 TAAGGCAGCAGCCCCAGGCAGCATTTAAGCGG(+ACTCAC)GATCCTTAGCAAGACAGAATCCAGCAAAACC NGG 10 8 17 8 24 0 100 5 10
    240605012233_glmu GAAAGGGTTTTGCTGGATTCTGTCTTGCTAAG(+ACTCAC)GATCCCGCTTAAATGCTGCCTGGGGCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605012232_4i86 GATCTCGTTTACCTCCTGTTTAGTGGGCTTTG(+ACTCAC)GATCCTCCCTGTTAGTTCTTCATGTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605012230_a6yc TAAGTAAAACCACATGAAGAACTAACAGGGAG(+ACTCAC)GATCCAAAGCCCACTAAACAGGAGGTAAACG NGG 10 8 17 8 24 0 100 5 10