Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012314_k6ju TGGACTTTCATAGGACATAAGCTTGAACCCCG(+ACTCAC)GTACCGCTCAGCAGAACAGTTGGGTTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605012312_e8mb AGGCACTGAGAACCCAACTGTTCTGCTGAGCG(+ACTCAC)GTACCGGGGTTCAAGCTTATGTCCTATGAAA NGG 10 8 17 8 24 0 100 5 10
    240605012311_y09s AGAACCTGCCACAGCTTAACACACCTTATCTG(+ACTCAC)GTACCTGTCTGAAAGCACTCCCTGGGCCTAT NGG 10 8 17 8 24 0 100 5 10
    240605012309_dury CATAGATAGGCCCAGGGAGTGCTTTCAGACAG(+ACTCAC)GTACCAGATAAGGTGTGTTAAGCTGTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605012308_zaxu ATTGTTAATGTGCCTTGCAACTGGGAGTCTAG(+ACTCAC)GTACCTAATGAAACTTTGTGACTTTAGCACA NGG 10 8 17 8 24 0 100 5 10
    240605012307_05bf GAACTTGTGCTAAAGTCACAAAGTTTCATTAG(+ACTCAC)GTACCTAGACTCCCAGTTGCAAGGCACATTA NGG 10 8 17 8 24 0 100 5 10
    240605012305_g0lx GCCTGGCCTGGGTGGGCCTGTGCTGTGCCTTG(+ACTCAC)GTACCATCTTCCCTCGATTTTCCTTGACCCT NGG 10 8 17 8 24 0 100 5 10
    240605012304_ezws GAAGGAGGGTCAAGGAAAATCGAGGGAAGATG(+ACTCAC)GTACCAAGGCACAGCACAGGCCCACCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605012302_wh2y CATGCATATTATTTATTTCTGTGCAAACTGTG(+ACTCAC)GATCCGTAACCAAGGTGAAGAAAGTTGGGAT NGG 10 8 17 8 24 0 100 5 10
    240605012301_kitr CAGAAATCCCAACTTTCTTCACCTTGGTTACG(+ACTCAC)GATCCACAGTTTGCACAGAAATAAATAATAT NGG 10 8 17 8 24 0 100 5 10