Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012228_oprx GCTGCATCTCAGATTGTGGGGGGTGGGAGGGG(+ACTCAC)GATCCTAACCTCAGGCACAATAGCATTCATA NGG 10 8 17 8 24 0 100 5 10
    240605012226_31jv CTGTTTATGAATGCTATTGTGCCTGAGGTTAG(+ACTCAC)GATCCCCCTCCCACCCCCCACAATCTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240605012225_4imd ATGTGTGCCCCAAATACTCATGCTGTTCAAAG(+ACTCAC)GATCCTCTTGTCTGCTTCGCATAGGGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605012224_5c4r CCACACTCCACCCTATGCGAAGCAGACAAGAG(+ACTCAC)GATCCTTTGAACAGCATGAGTATTTGGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605012222_ewqg TAATAAGTCCGTTAAAAGCAGAGCCAGGACTG(+ACTCAC)GATCCCGAAAGAATAGGCTTTTGAAACAATC NGG 10 8 17 8 24 0 100 5 10
    240605012221_3iyp CATGTGATTGTTTCAAAAGCCTATTCTTTCGG(+ACTCAC)GATCCAGTCCTGGCTCTGCTTTTAACGGACT NGG 10 8 17 8 24 0 100 5 10
    240605012220_541l TCACATCATTTTCTTCCAGTTATTGGCTACAG(+ACTCAC)GATCCAAGGGGAAGGTTTTCTGCTTCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605012217_is1w CTGTGAGCTGGAAGCAGAAAACCTTCCCCTTG(+ACTCAC)GATCCTGTAGCCAATAACTGGAAGAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240605012215_gp5m ATGTGCCAAGGCTCCAACAGACAAGATTATTG(+ACTCAC)GTACCTCTTTGTAGGGCAGATTTATAGTTAT NGG 10 8 17 8 24 0 100 5 10
    240605012214_zpv1 GAATTATAACTATAAATCTGCCCTACAAAGAG(+ACTCAC)GTACCAATAATCTTGTCTGTTGGAGCCTTGG NGG 10 8 17 8 24 0 100 5 10