Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012125_ep8x AGGTAGAGCCCTGAGAGTTCCCAGCAGAGTGG(+ACTCAC)GATCCAGACTTTGCACAGAAATGATCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605012123_bbi7 GGTGACCTGAGATCATTTCTGTGCAAAGTCTG(+ACTCAC)GATCCCACTCTGCTGGGAACTCTCAGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605012122_p6s9 CCGGCCTCCTCCCCTCACACACTCTGGACTTG(+ACTCAC)GTACCGAATAATCCCGTGTGGTCCTAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605012121_3tt3 GTGCTTCAGCTAGGACCACACGGGATTATTCG(+ACTCAC)GTACCAAGTCCAGAGTGTGTGAGGGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605012119_9937 GTTACTTTAAGTCCTGAGATGCAGGTTCCCTG(+ACTCAC)GTACCATTGAGTTGCTGCTACTAAAAGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605012117_o8gv GCATGTGAGCTTTTAGTAGCAGCAACTCAATG(+ACTCAC)GTACCAGGGAACCTGCATCTCAGGACTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605012116_v2iy AGATACAGCTGTCCCGTGGAGCAGCAGGCATG(+ACTCAC)GATCCCCTCCATCCTTGGGCTTCCCGGGCCC NGG 10 8 17 8 24 0 100 5 10
    240605012114_dolj CGCAGGGGCCCGGGAAGCCCAAGGATGGAGGG(+ACTCAC)GATCCATGCCTGCTGCTCCACGGGACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605012113_sxgi GCCTATTTTGTGATGTGATGACCTTCAAATTG(+ACTCAC)GATCCCGTGGCTTGTCCTCTCTGAAGGCCGG NGG 10 8 17 8 24 0 100 5 10
    240605012111_edr8 GGGCTCCGGCCTTCAGAGAGGACAAGCCACGG(+ACTCAC)GATCCAATTTGAAGGTCATCACATCACAAAA NGG 10 8 17 8 24 0 100 5 10