Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012055_qbn1 AGAATTTACCTTTACAATTTATTTTCAAACTG(+ACTCAC)GATCCATTCAATTGTCATGTATGTGTGCCTA NGG 10 8 17 8 24 0 100 5 10
    240605012054_3n1u CACAGTAGGCACACATACATGACAATTGAATG(+ACTCAC)GATCCAGTTTGAAAATAAATTGTAAAGGTAA NGG 10 8 17 8 24 0 100 5 10
    240605012052_sv5o TTTTCAAGTCAAAATTTTAGGACAAATTTTAG(+ACTCAC)GATCCTGGTTATCATATTATAGTATTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605012051_8fwz CAAAATCCTGAATACTATAATATGATAACCAG(+ACTCAC)GATCCTAAAATTTGTCCTAAAATTTTGACTT NGG 10 8 17 8 24 0 100 5 10
    240605012049_vb6w TTTTAATAGAGAAACAGGACACTGATTTTGAG(+ACTCAC)GATCCTGACTCCAAAGCTAATGTCCTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240605012048_gemd AGACTGAAGAAGGACATTAGCTTTGGAGTCAG(+ACTCAC)GATCCTCAAAATCAGTGTCCTGTTTCTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605012046_2s17 ACCAAACTCTGAAGGAAACACTGCTGAGGAAG(+ACTCAC)GTACCCTAGTGGATAAGGTTAGATTTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605012044_j68v CAAGGCCCTGAAATCTAACCTTATCCACTAGG(+ACTCAC)GTACCTTCCTCAGCAGTGTTTCCTTCAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605012043_8gqi AAGCCATTTTGTTGAGGGAGGGGCTTTTCCTG(+ACTCAC)GTACCTATCTCCTAAAGGGGTTACTTTCGAA NGG 10 8 17 8 24 0 100 5 10
    240605012042_3dal GTGAATTCGAAAGTAACCCCTTTAGGAGATAG(+ACTCAC)GTACCAGGAAAAGCCCCTCCCTCAACAAAAT NGG 10 8 17 8 24 0 100 5 10